ArticleThe Plant cell2001
A harpin binding site in tobacco plasma membranes mediates activation of the pathogenesis-related gene HIN1 independent of extracellular calcium but dependent on mitogen-activated protein kinase activity.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 216 citations in OpenAlex.
- Systematic Screening of Host Interactors forPlants (Basel, Switzerland) · 2026Article
- Refractory Behavior in Plant Cells-Calcium Signaling Induced by Biotic Stress.Plants (Basel, Switzerland) · 2026Review
- The engineered Immunoprotein enhance broad-spectrum disease resistance in solanaceous crops.BMC plant biology · 2026Article
- Secreted Glycoside Hydrolase BcGH61 From Botrytis cinerea Induces Cell Death by the Apoplastic Location and Triggers Intracellular Immune Perception.Molecular plant pathology · 2026Article
- The Harpin-Induced Hypersensitive Reaction in Nicotiana tabacum Requires Wall-Associated Kinase 2.Molecular plant pathology · 2025Article
- The Past, Present, and Future of Plant Activators Targeting the Salicylic Acid Signaling Pathway.Genes · 2024Review
- Cloning of the SoybeanPlants (Basel, Switzerland) · 2023Article
- Characteristics, Roles and Applications of Proteinaceous Elicitors from Pathogens in Plant Immunity.Life (Basel, Switzerland) · 2023Review
- The heterologous expression of conserved Glycine max (soybean) mitogen activated protein kinase 3 (MAPK3) paralogs suppresses Meloidogyne incognita parasitism in Gossypium hirsutum (upland cotton).Transgenic research · 2022Article
- The Promoter Analysis ofPlants (Basel, Switzerland) · 2022Article
- Bacterial extracellular matrix as a natural source of biotechnologically multivalent materials.Computational and structural biotechnology journal · 2021Review
- Research on the Interaction Mechanism Between α Mino-Phosphonate Derivative Q-R and Harpin-Binding Protein 1 in Tobacco (Frontiers in microbiology · 2021Article
- CGTase, a novel antimicrobial protein from Bacillus cereus YUPP-10, suppresses Verticillium dahliae and mediates plant defence responses.Molecular plant pathology · 2021Article
- Coiled-Coil N21 of Hpa1 inInternational journal of molecular sciences · 2020Article
- Elicitor and Receptor Molecules: Orchestrators of Plant Defense and Immunity.International journal of molecular sciences · 2020Review
- TheFrontiers in plant science · 2020Article
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- Apoplastic invasion patterns triggering plant immunity: plasma membrane sensing at the frontline.Molecular plant pathology · 2019Review
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation.Journal of experimental botany · 2019Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Harpin from the bean halo-blight pathogen Pseudomonas syringae pv phaseolicola (harpin(Psph)) elicits the hypersensitive response and the accumulation of pathogenesis-related gene transcripts in the nonhost plant tobacco. Here, we report the characterization of a nonproteinaceous binding site for harpin(Psph) in tobacco plasma membranes, which is assumed to mediate the activation of plant defense responses in a receptor-like manner. Binding of 125I-harpin(Psph) to tobacco microsomal membranes (dissociation constant = 425 nM) and protoplasts (dissociation constant = 380 nM) was specific, reversible, and saturable. A close correlation was found between the abilities of harpin(Psph) fragments to elicit the transcript accumulation of the pathogenesis-related tobacco gene HIN1 and to compete for binding of 125I-harpin(Psph) to its binding site. Another elicitor of the hypersensitive response and HIN1 induction in tobacco, the Phytophthora megasperma-derived beta-elicitin beta-megaspermin, failed to bind to the putative harpin(Psph) receptor. In contrast to activation by beta-megaspermin, harpin(Psph)-induced activation of the 48-kD salicylic acid-responsive mitogen-activated protein kinase (MAPK) and HIN1 transcript accumulation were independent of extracellular calcium. Moreover, use of the MAPK kinase inhibitor U0126 revealed that MAPK activity was essential for pathogenesis-related gene expression in harpin(Psph)-treated tobacco cells. Thus, a receptor-mediated MAPK-dependent signaling pathway may mediate the activation of plant defense responses induced by harpin(Psph).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.