Evidence map›Paper›PMID 11336651›Full record

ArticleThe Biochemical journal2001

Activation of store-mediated calcium entry by secretion-like coupling between the inositol 1,4,5-trisphosphate receptor type II and human transient receptor potential (hTrp1) channels in human platelets.

J A Rosado, S O Sage

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.

  1. Pooled it
  2. Proteins Interacting with STIM1 and Store-Operated CaProgress in molecular and subcellular biology · 2021
    Review
  3. Store-Operated CaInternational journal of molecular sciences · 2018
    Review
  4. Article
  5. The functions of store-operated calcium channels.Biochimica et biophysica acta. Molecular cell research · 2017
    Review
  6. Platelet receptor signaling in thrombus formation.Journal of molecular medicine (Berlin, Germany) · 2011
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

J A RosadoDepartment of Physiology, University of Cambridge, Downing St., Cambridge CB2 3EG, UK.
S O Sage
University of Cambridge · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Physical coupling between inositol 1,4,5-trisphosphate (IP(3)) receptors and transient receptor potential (Trp) channels has been demonstrated in both transfected and normal cells as a candidate mechanism for the activation of store-mediated Ca(2+) entry (SMCE). We have investigated the properties of the coupling between the type II IP(3) receptor and naturally expressed human Trp1 (hTrp1) in human platelets. Treatment with xestospongin C, an inhibitor of IP(3) receptor function, abolished SMCE and coupling between the IP(3) receptor and hTrp1. The coupling was activated by depletion of the intracellular Ca(2+) stores, and was reversed by refilling of the stores. We have also examined the role of actin filaments in the activation and maintenance of the coupling. Stabilization of the cortical actin network with jasplakinolide prevented the coupling, indicating that, as with secretion, the actin filaments at the cell periphery act as a negative clamp which prevents constitutive coupling. In addition, the actin cytoskeleton plays a positive role, since disruption of the actin network inhibited the coupling when the Ca(2+) stores were depleted. These results provide strong evidence for the activation of SMCE by a secretion-like coupling mechanism involving a reversible association between IP(3) receptors and hTrp1 in normal human cells.

Indexed as

ActinsBiological TransportBlood PlateletsCalciumCalcium ChannelsCytochalasin DCytoskeletonEnzyme InhibitorsHumansInositol 1,4,5-Trisphosphate ReceptorsMacrocyclic CompoundsOxazolesProtein BindingReceptor Cross-TalkReceptors, Cytoplasmic and NuclearTRPC Cation ChannelsActinsCalciumCalcium ChannelsCytochalasin DEnzyme InhibitorsInositol 1,4,5-Trisphosphate ReceptorsITPR1 protein, humanMacrocyclic CompoundsOxazolesReceptors, Cytoplasmic and Nucleartransient receptor potential cation channel, subfamily C, member 1TRPC Cation Channelsxestospongin A

Identifiers

PMID11336651
PMCPMC1221827
OpenAlexW2007092678

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.