ArticleThe Biochemical journal2001
Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Localization of the tubby domain, a PI(4,5)P2 biosensor, to E-Syt3-rich endoplasmic reticulum-plasma membrane junctions.Journal of cell science · 2023Article
- Regulation of longevity by depolarization-induced activation of PLC-β-IPProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Blue light-triggered photochemistry and cytotoxicity of retinal.Cellular signalling · 2020Article
- Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling.Analytical and bioanalytical chemistry · 2019Review
- Expression variation and covariation impair analog and enable binary signaling control.Molecular systems biology · 2018Article
- In vivo tracking of phosphoinositides in Drosophila photoreceptors.Journal of cell science · 2015Article
- Caveolin-1 alters the pattern of cytoplasmic Ca2+ oscillations and Ca2+-dependent gene expression by enhancing leukotriene receptor desensitization.The Journal of biological chemistry · 2014Article
- Inositol 1,4,5-trisphosphate receptor-mediated calcium release in Purkinje cells: from molecular mechanism to behavior.Cerebellum (London, England) · 2011Review
- Inositol 1,4,5-trisphosphate receptor subtype-specific regulation of calcium oscillations.Neurochemical research · 2011Review
- Phase-locked signals elucidate circuit architecture of an oscillatory pathway.PLoS computational biology · 2010Article
- Odorant-stimulated phosphoinositide signaling in mammalian olfactory receptor neurons.Cellular signalling · 2010Article
- Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative study.BMC cell biology · 2009Article
- Visualization of cellular phosphoinositide pools with GFP-fused protein-domains.Current protocols in cell biology · 2009Article
- Live cell imaging of phosphoinositides with expressed inositide binding protein domains.Methods (San Diego, Calif.) · 2008Article
- Temporal profiling of changes in phosphatidylinositol 4,5-bisphosphate, inositol 1,4,5-trisphosphate and diacylglycerol allows comprehensive analysis of phospholipase C-initiated signalling in single neurons.Journal of neurochemistry · 2008Article
- Selective regulation of H1 histamine receptor signaling by G protein-coupled receptor kinase 2 in uterine smooth muscle cells.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Monitoring changes in membrane phosphatidylinositol 4,5-bisphosphate in living cells using a domain from the transcription factor tubby.The Journal of physiology · 2008Article
- Acetylcholine-induced Ca2+ oscillations are modulated by a Ca2+ regulation of InsP3R2 in rat portal vein myocytes.Pflugers Archiv : European journal of physiology · 2008Article
- Target-specific PIP(2) signalling: how might it work?The Journal of physiology · 2007Article
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pleckstrin homology domain of phospholipase Cdelta1 (PH(PLCdelta)) binds Ins(1,4,5)P(3) and PtdIns(4,5)P(2) specifically, and can be used to detect changes in Ins(1,4,5)P(3) in single cells. A fusion construct of PH(PLCdelta) and enhanced green fluorescent protein (EGFP-PH(PLCdelta)) associates with the plasma membrane due to its association with PtdIns(4,5)P(2). However, PH(PLCdelta) has greater affinity for Ins(1,4,5)P(3) than PtdIns(4,5)P(2), and translocates to the cytosol as Ins(1,4,5)P(3) levels rise. Prolonged activation of group I metabotropic glutamate receptor 1alpha expressed in Chinese-hamster ovary cells or endogenous M(3) muscarinic receptors in SH-SY5Y neuroblastoma cells gave an initial transient peak in translocation, followed by a sustained plateau phase. This closely followed changes in cell population Ins(1,4,5)P(3) mass, but not PtdIns(4,5)P(2) levels, which decreased monophasically, as determined by radioreceptor assay. Translocation thus provides a real-time method to follow increases in Ins(1,4,5)P(3). Graded changes in Ins(1,4,5)P(3) in Chinese-hamster ovary-lac-mGlu1alpha cells could be detected with increasing glutamate concentrations, and dual loading with fura 2 and EGFP-PH(PLCdelta) showed that changes in intracellular Ca(2+) concentration closely paralleled Ins(1,4,5)P(3) production. Moreover, Ins(1,4,5)P(3) accumulation and intracellular Ca(2+) mobilization within single cells is graded in nature and dependent on both agonist concentration and receptor density.
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