ArticleThe Journal of molecular diagnostics : JMD2001
Tissue microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) is an effective method to analyze genetic aberrations in invasive tumors.
Article in The Journal of molecular diagnostics : JMD, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular analysis of H&E- and Papanicolau-stained samples-systematic review.Histochemistry and cell biology · 2020Pooled it
- Clinical and histopathological analyses of VEGF receptors peptide vaccine in patients with primary glioblastoma - a case series.BMC cancer · 2020Trial
- Factors involved in maintaining Karnofsky Performance Status (≥ 50%) in glioblastoma, IDH-wildtype patients treated with temozolomide and radiotherapy.Scientific reports · 2025Article
- Clinical, histopathological and molecular risk factors for recurrence of pilocytic astrocytomas: brainstem/spinal location, nestin expression and gain of 7q and 19 are associated with early tumor recurrence.Brain tumor pathology · 2023Article
- Magnetic resonance imaging texture analyses in lower-grade gliomas with a commercially available software: correlation of apparent diffusion coefficient and T2 skewness with 1p/19q codeletion.Neurosurgical review · 2020Article
- Imaging scoring systems for preoperative molecular diagnoses of lower-grade gliomas.Neurosurgical review · 2019Article
- A pilot study of peptide vaccines for VEGF receptor 1 and 2 in patients with recurrent/progressive high grade glioma.Oncotarget · 2018Article
- Comprehensive genetic characterization of rosette-forming glioneuronal tumors: independent component analysis by tissue microdissection.Brain pathology (Zurich, Switzerland) · 2018Article
- Molecular-genetic and clinicopathological prognostic factors in patients with gliomas showing total 1p19q loss: gain of chromosome 19p and histological grade III negatively correlate with patient's prognosis.Journal of neuro-oncology · 2017Article
- World Health Organization grade II-III astrocytomas consist of genetically distinct tumor lineages.Cancer science · 2016Article
- Upfront chemotherapy and subsequent resection for molecularly defined gliomas.Journal of neuro-oncology · 2015Observational
- Molecular-genetic and clinical characteristics of gliomas with astrocytic appearance and total 1p19q loss in a single institutional consecutive cohort.Oncotarget · 2015Article
- Radiological features of supratentorial gliomas are associated with their genetic aberrations.Neurosurgical review · 2014Article
- Comparison of whole genome amplification methods for analysis of DNA extracted from microdissected early breast lesions in formalin-fixed paraffin-embedded tissue.ISRN oncology · 2012Article
- Aggressive large cell medulloblastoma extending to the extracranial region in brain-dead state.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2011Article
- Rapid spontaneous regression of multicentric infantile myofibromatosis in the posterior fossa and lumbar vertebra.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2011Article
- Meningioma of the cerebellopontine angle in identical twins: a case report.Journal of neuro-oncology · 2010Article
- Early malignant transformation of a petroclival meningothelial meningioma.Neurosurgical review · 2009Article
- Genetic characterization of adult infratentorial gliomas.Journal of neuro-oncology · 2009Article
- Predominant expression of OLIG2 over ID2 in oligodendroglial tumors.Virchows Archiv : an international journal of pathology · 2007Article
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5 authors.
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Abstract
We amplified various amounts of DNA derived from frozen SF210 and U251NCI human glioblastoma cells, carried out comparative genomic hybridization (CGH) using degenerate oligonucleotide primed-PCR (DOP-PCR) products as test probes, and compared results to analyses performed with probes prepared by standard nick translation. Next we extracted DNA from hematoxylin-eosin (HE)- and methyl green (MG)-stained, microdissected sections of formalin-fixed and paraffin-embedded U251NCI cells, amplified and labeled it by DOP-PCR, and subjected it to CGH. Finally, we used the same methods in multiple samples from a single human mixed glioma tissue. DOP-PCR products from 50 pg to 250 ng of DNA were equally effective in generating the same CGH profiles as the standard method. DOP-PCR products from microdissected pieces of MG-stained cells were effective probes for CGH, but HE-stained samples were not desirable. As the proportion of HE-stained sample increased, CGH profiles deteriorated. DOP-PCR products from microdissected pieces of MG-stained paraffin sections of glioma tissue produced CGH profiles compatible with their histological features. CGH performed with DOP-PCR products from microdissected paraffin blocks allows for the accurate investigation of the cytogenetic characteristics from invasive tumors and of cytogenetic heterogeneity within neoplastic tissue.
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