ArticleGenetics2001
DNA breaks in hypermutating immunoglobulin genes: evidence for a break-and-repair pathway of somatic hypermutation.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- DNA Breaks in Ig V Regions Are Predominantly Single Stranded and Are Generated by UNG and MSH6 DNA Repair Pathways.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Initiation of homologous recombination at DNA nicks.Nucleic acids research · 2018Review
- Opinion: uracil DNA glycosylase (UNG) plays distinct and non-canonical roles in somatic hypermutation and class switch recombination.International immunology · 2014Review
- Non-conservative homologous recombination in human B lymphocytes is promoted by activation-induced cytidine deaminase and transcription.Nucleic acids research · 2008Article
- Known components of the immunoglobulin A:T mutational machinery are intact in Burkitt lymphoma cell lines with G:C bias.Molecular immunology · 2007Article
- Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation.The Journal of experimental medicine · 2007Article
- Codon insertion and deletion functions as a somatic diversification mechanism in human antibody repertoires.Biology direct · 2006Article
- AID to overcome the limitations of genomic information by introducing somatic DNA alterations.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2006Review
- Evaluation of molecular models for the affinity maturation of antibodies: roles of cytosine deamination by AID and DNA repair.Chemical reviews · 2006Review
- NHEJ-deficient DT40 cells have increased levels of immunoglobulin gene conversion: evidence for a double strand break intermediate.Nucleic acids research · 2006Article
- Hepatitis C virus E2-CD81 interaction induces hypermutation of the immunoglobulin gene in B cells.Journal of virology · 2005Article
- DNA cleavage in immunoglobulin somatic hypermutation depends on de novo protein synthesis but not on uracil DNA glycosylase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Hepatitis C virus induces a mutator phenotype: enhanced mutations of immunoglobulin and protooncogenes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast.The EMBO journal · 2004Article
- Transcription-coupled mutagenesis by the DNA deaminase AID.Genome biology · 2004Review
- Overexpression of human DNA polymerase mu (Pol mu) in a Burkitt's lymphoma cell line affects the somatic hypermutation rate.Nucleic acids research · 2004Article
- Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The roles of REV3 and RAD57 in double-strand-break-repair-induced mutagenesis of Saccharomyces cerevisiae.Genetics · 2002Article
- Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The function of AID in somatic mutation and class switch recombination: upstream or downstream of DNA breaks.The Journal of experimental medicine · 2002Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
To test the hypothesis that immunoglobulin gene hypermutation in vivo employs a pathway in which DNA breaks are introduced and subsequently repaired to produce mutations, we have used a PCR-based assay to detect and identify single-strand DNA breaks in lambda1 genes of actively hypermutating primary murine germinal center B cells. We find that there is a two- to threefold excess of breaks in lambda1 genes of hypermutating B cells, relative to nonhypermutating B cells, and that 1.3% of germinal center B cells contain breaks in the lambda1 gene that are associated with hypermutation. Breaks were found in both top and bottom DNA strands and were localized to the region of lambda1 that actively hypermutates, but duplex breaks accounted for only a subset of breaks identified. Almost half of the breaks in hypermutating B cells occurred at hotspots, sites at which two or more independent breaks were identified. Breaksite hotspots were associated with characteristic sequence motifs: a pyrimidine-rich motif, either RCTYT or CCYC; and RGYW, a sequence motif associated with hypermutation hotspots. The sequence motifs identified at breaksite hotspots should inform the design of substrates for characterization of activities that participate in the hypermutation pathway.
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