ArticleGenetics2001
Long-range repression by multiple polycomb group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.Genes & development · 2015Article
- Chromatin regulation: how complex does it get?Epigenetics · 2014Review
- Interactions with RNA direct the Polycomb group protein SCML2 to chromatin where it represses target genes.eLife · 2014Article
- What are memories made of? How Polycomb and Trithorax proteins mediate epigenetic memory.Nature reviews. Molecular cell biology · 2014Review
- The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.Genetics · 2013Article
- Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.PloS one · 2012Article
- Small ubiquitin-like modifier (SUMO) conjugation impedes transcriptional silencing by the polycomb group repressor Sex Comb on Midleg.The Journal of biological chemistry · 2011Article
- Comparative analysis of chromatin binding by Sex Comb on Midleg (SCM) and other polycomb group repressors at a Drosophila Hox gene.Molecular and cellular biology · 2010Article
- Recruitment of polycomb complexes: a role for SCM.Molecular and cellular biology · 2010Article
- MBT domain proteins in development and disease.Seminars in cell & developmental biology · 2010Review
- Sterile alpha motif domain-mediated self-association plays an essential role in modulating the activity of the Drosophila ETS family transcriptional repressor Yan.Molecular and cellular biology · 2010Article
- Cloning and characterization of mr-s, a novel SAM domain protein, predominantly expressed in retinal photoreceptor cells.BMC developmental biology · 2006Article
- Article
- Requirement for sex comb on midleg protein interactions in Drosophila polycomb group repression.Genetics · 2004Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A tethering assay was developed to study the effects of Polycomb group (PcG) proteins on gene expression in vivo. This system employed the Su(Hw) DNA-binding domain (ZnF) to direct PcG proteins to transposons that carried the white and yellow reporter genes. These reporters constituted naive sensors of PcG effects, as bona fide PcG response elements (PREs) were absent from the constructs. To assess the effects of different genomic environments, reporter transposons integrated at nearly 40 chromosomal sites were analyzed. Three PcG fusion proteins, ZnF-PC, ZnF-SCM, and ZnF-ESC, were studied, since biochemical analyses place these PcG proteins in distinct complexes. Tethered ZnF-PcG proteins repressed white and yellow expression at the majority of sites tested, with each fusion protein displaying a characteristic degree of silencing. Repression by ZnF-PC was stronger than ZnF-SCM, which was stronger than ZnF-ESC, as judged by the percentage of insertion lines affected and the magnitude of the conferred repression. ZnF-PcG repression was more effective at centric and telomeric reporter insertion sites, as compared to euchromatic sites. ZnF-PcG proteins tethered as far as 3.0 kb away from the target promoter produced silencing, indicating that these effects were long range. Repression by ZnF-SCM required a protein interaction domain, the SPM domain, which suggests that this domain is not primarily used to direct SCM to chromosomal loci. This targeting system is useful for studying protein domains and mechanisms involved in PcG repression in vivo.
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