ArticleRNA (New York, N.Y.)2001
Translational control by delayed RNA folding: identification of the kinetic trap.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Incorporating age and delay into models for biophysical systems.Physical biology · 2021Article
- A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA.eLife · 2019Article
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
- Type I Toxin-Antitoxin Systems: Regulating Toxin Expression via Shine-Dalgarno Sequence Sequestration and Small RNA Binding.Microbiology spectrum · 2018Review
- The HIV-1 Rev response element (RRE) adopts alternative conformations that promote different rates of virus replication.Nucleic acids research · 2015Article
- Four RNA families with functional transient structures.RNA biology · 2015Article
- Article
- Transient RNA structure features are evolutionarily conserved and can be computationally predicted.Nucleic acids research · 2013Article
- Regulation of translation initiation by RNA binding proteins.Annual review of microbiology · 2009Review
- Distinct contribution of electrostatics, initial conformational ensemble, and macromolecular stability in RNA folding.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Encoding folding paths of RNA switches.Nucleic acids research · 2007Article
- Complete genome sequence of the broad host range single-stranded RNA phage PRR1 places it in the Levivirus genus with characteristics shared with Alloleviviruses.Journal of virology · 2006Article
- Structural constraints and mutational bias in the evolutionary restoration of a severe deletion in RNA phage MS2.Journal of molecular evolution · 2006Article
- Structural parameters affecting the kinetics of RNA hairpin formation.Nucleic acids research · 2006Article
- Discontinuous subgenomic RNA synthesis in arteriviruses is guided by an RNA hairpin structure located in the genomic leader region.Journal of virology · 2005Article
- RNA stem-loop enhanced expression of previously non-expressible genes.Nucleic acids research · 2004Article
- A pH-jump approach for investigating secondary structure refolding kinetics in RNA.Nucleic acids research · 2002Article
- An insight into the possible mechanism of working of two-cistronic gene expression systems and rational designing of newer systems.Journal of biosciences · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maturation or A-protein gene of single-stranded RNA phage MS2 is preceded by a 130-nt long untranslated leader. When MS2 RNA folding is at equilibrium, the gene is untranslatable because the leader adopts a well-defined cloverleaf structure in which the Shine-Dalgarno (SD) sequence of the maturation gene is taken up in long-distance base pairing with an upstream complementary sequence (UCS). Synthesis of the A-protein takes place transiently while the RNA is synthesized from the minus strand. This requires that formation of the inhibitory cloverleaf is slow. In vitro, the folding delay was on the order of minutes. Here, we present evidence that this postponed folding is caused by the formation of a metastable intermediate. This intermediate is a small local hairpin that contains the UCS in its loop, thereby preventing or slowing down its pairing with the SD sequence. Mutants in which the small hairpin could not be formed made no detectable amounts of A-protein and were barely viable. Apparently, here the cloverleaf formed quicker than ribosomes could bind. On the other hand, mutants in which the small intermediary hairpin was stabilized produced more A-protein than wild type and were viable. One hardly growing mutant that could not form the metastable hairpin and did not make detectable amounts of A-protein was evolved. The emerging pseudo-revertant had selected two second site repressor mutations that allowed reconstruction of a variant of the metastable intermediate. The pseudo-revertant had also regained the capacity to produce the A-protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.