ArticleRNA (New York, N.Y.)2001
Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 120 citations in OpenAlex.
- Therapeutic Nonsense Suppression Modalities: From Small Molecules to Nucleic Acid-Based Approaches.Biomedicines · 2024Review
- Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells.Molecular cell · 2022Article
- Positive natural selection of N6-methyladenosine on the RNAs of processed pseudogenes.Genome biology · 2021Article
- Suppression of Nonsense Mutations by New Emerging Technologies.International journal of molecular sciences · 2020Review
- Recurrent SRSF2 mutations in MDS affect both splicing and NMD.Genes & development · 2020Article
- Phylogenetic and genomic analyses of the ribosomal oxygenases Riox1 (No66) and Riox2 (Mina53) provide new insights into their evolution.BMC evolutionary biology · 2018Article
- Article
- RNA biology of disease-associated microsatellite repeat expansions.Acta neuropathologica communications · 2017Review
- CRISPRtools: a flexible computational platform for performing CRISPR/Cas9 experiments in the mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
- Mutations in Epigenetic Regulation Genes Are a Major Cause of Overgrowth with Intellectual Disability.American journal of human genetics · 2017Article
- Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.International journal of biological sciences · 2017Review
- The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.Molecular and cellular biology · 2016Article
- Mechanism, factors, and physiological role of nonsense-mediated mRNA decay.Cellular and molecular life sciences : CMLS · 2015Review
- Retroviral strategy to stabilize viral RNA.Current opinion in microbiology · 2014Review
- Organizing principles of mammalian nonsense-mediated mRNA decay.Annual review of genetics · 2013Review
- Genome-wide activation of latent donor splice sites in stress and disease.Nucleic acids research · 2012Article
- Translation drives mRNA quality control.Nature structural & molecular biology · 2012Article
- Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g.The Journal of biological chemistry · 2012Article
- Yeast mRNA cap-binding protein Cbc1/Sto1 is necessary for the rapid reprogramming of translation after hyperosmotic shock.Molecular biology of the cell · 2012Article
- Application of a novel strategy of engineering conditional alleles to a single exon gene, Sox2.PloS one · 2012Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Nonsense-mediated decay (NMD), also called mRNA surveillance, is an evolutionarily conserved pathway that degrades mRNAs that prematurely terminate translation. To date, the pathway in mammalian cells has been shown to depend on the presence of a cis-acting destabilizing element that usually consists of an exon-exon junction generated by the process of pre-mRNA splicing. Whether or not mRNAs that derive from naturally intronless genes, that is, mRNAs not formed by the process of splicing, are also subject to NMD has yet to be investigated. The possibility of NMD is certainly reasonable considering that mRNAs of Saccharomyces cerevisiae are subject to NMD even though most derive from naturally intronless genes. In fact, mRNAs of S. cerevisiae generally harbor a loosely defined splicing-independent destabilizing element that has been proposed to function in NMD analogously to the spliced exon-exon junction of mammalian mRNAs. Here, we demonstrate that nonsense codons introduced into naturally intronless genes encoding mouse heat shock protein 70 or human histone H4 fail to elicit NMD. Failure is most likely because each mRNA lacks a cis-acting destabilizing element, because insertion of a spliceable intron a sufficient distance downstream of a nonsense codon within either gene is sufficient to elicit NMD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.