ArticleRNA (New York, N.Y.)2001
The effect of cytidine on the structure and function of an RNA ligase ribozyme.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- A ribozyme ligase that requires a 3' terminal phosphate on its RNA substrate.Nature communications · 2026Article
- Assembly of catalytic complexes from randomized oligonucleotides.Science advances · 2025Article
- On an RNA-Membrane Protogenome.Life (Basel, Switzerland) · 2025Article
- Effects of lipid membranes on RNA catalytic activity and stability.Biology of the cell · 2025Article
- Development of Allosteric Ribozymes for ATP and l-Histidine Based on the R3C Ligase Ribozyme.Life (Basel, Switzerland) · 2024Article
- Origin of ribonucleotide recognition motifs through ligand mimicry at early earth.RNA biology · 2024Review
- A ribozyme that uses lanthanides as cofactor.Nucleic acids research · 2023Article
- Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution.PLoS genetics · 2023Article
- Minimal RNA self-reproduction discovered from a random pool of oligomers.Chemical science · 2023Article
- Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit.Biology · 2023Article
- Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations.Nature communications · 2023Article
- Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions.Life (Basel, Switzerland) · 2022Article
- An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination.Nature chemistry · 2022Article
- Lipid membranes modulate the activity of RNA through sequence-dependent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Self-Reproduction and Darwinian Evolution in Autocatalytic Chemical Reaction Systems.Life (Basel, Switzerland) · 2021Review
- ZnCommunications biology · 2021Article
- A combinatorial method to isolate short ribozymes from complex ribozyme libraries.Nucleic acids research · 2020Article
- The 3-Minihelix tRNA Evolution Theorem.Journal of molecular evolution · 2020Article
- Evolution of Life on Earth: tRNA, Aminoacyl-tRNA Synthetases and the Genetic Code.Life (Basel, Switzerland) · 2020Review
- Nucleic Acid Catalysis under Potential Prebiotic Conditions.Chemistry, an Asian journal · 2020Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cytidine-free ribozyme with RNA ligase activity was obtained by in vitro evolution, starting from a pool of random-sequence RNAs that contained only guanosine, adenosine, and uridine. This ribozyme contains 74 nt and catalyzes formation of a 3',5'-phosphodiester linkage with a catalytic rate of 0.016 min(-1). The RNA adopts a simple secondary structure based on a three-way junction motif, with ligation occurring at the end of a stem region located several nucleotides away from the junction. Cytidine was introduced to the cytidine-free ribozyme in a combinatorial fashion and additional rounds of in vitro evolution were carried out to allow the molecule to adapt to this added component. The resulting cytidine-containing ribozyme formed a 3',5' linkage with a catalytic rate of 0.32 min(-1). The improved rate of the cytidine-containing ribozyme was the result of 12 mutations, including seven added cytidines, that remodeled the internal bulge loops located adjacent to the three-way junction and stabilized the peripheral stem regions.
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