ArticleProceedings of the National Academy of Sciences of the United States of America2001
Differential fluorescence labeling of cysteinyl clusters uncovers high tissue levels of thionein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 141 citations in OpenAlex.
- Article
- Zinc-mediated dynamics of CD4/CD8α co-receptors and Lck kinase: implications for zinc homeostasis, immune response, and biotechnological innovations.Metallomics : integrated biometal science · 2025Review
- Copper induces neuron-sparing, ferredoxin 1-independent astrocyte toxicity mediated by oxidative stress.Journal of neurochemistry · 2023Article
- From methodological limitations to the function of metallothioneins - a guide to approaches for determining weak, moderate, and tight affinity zinc sites.Metallomics : integrated biometal science · 2023Article
- Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of zinc metallothionein-2 species.Chemical communications (Cambridge, England) · 2023Article
- An Integrated Mass Spectrometry and Molecular Dynamics Simulations Approach Reveals the Spatial Organization Impact of Metal-Binding Sites on the Stability of Metal-Depleted Metallothionein-2 Species.Journal of the American Chemical Society · 2021Article
- Mass Spectrometry-Based Structural Analysis of Cysteine-Rich Metal-Binding Sites in Proteins with MetaOdysseus R Software.Journal of proteome research · 2021Article
- AgAnalytical chemistry · 2020Article
- Metallothionein 2A gene polymorphisms in relation to diseases and trace element levels in humans.Arhiv za higijenu rada i toksikologiju · 2020Review
- Mercury(II) Binding to Metallothionein in Mytilus edulis revealed by High Energy-Resolution XANES Spectroscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Metallothionein: An Aggressive Scavenger-The Metabolism of Rhodium(II) Tetraacetate (RhACS omega · 2018Article
- Collision-Induced Unfolding of Partially Metalated Metallothionein-2A: Tracking Unfolding Reactions of Gas-Phase Ions.Analytical chemistry · 2018Article
- Proteomic High Affinity ZnInternational journal of molecular sciences · 2017Review
- The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism.International journal of molecular sciences · 2017Review
- Selective cysteine modification of metal-free human metallothionein 1a and its isolated domain fragments: Solution structural properties revealed via ESI-MS.Protein science : a publication of the Protein Society · 2017Article
- Oxidative stress and related biomarkers in cupric and cuprous chloride-treated rainbow trout.Environmental science and pollution research international · 2017Article
- Zinc as Allosteric Ion Channel Modulator: Ionotropic Receptors as Metalloproteins.International journal of molecular sciences · 2016Review
- Metallothioneins, unconventional proteins from unconventional animals: a long journey from nematodes to mammals.Biomolecules · 2014Review
- Metallothionein (MT) -I and MT-II expression are induced and cause zinc sequestration in the liver after brain injury.PloS one · 2012Article
- Redox regulation of intracellular zinc: molecular signaling in the life and death of neurons.Antioxidants & redox signaling · 2011Review
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The isolation of thionein (T) from tissues has not been reported heretofore. T contains 20 cysteinyl residues that react with 7-fluorobenz-2-oxa-1,3-diazole-4-sulfonamide to form fluorescent adducts. In metallothionein (MT) the cysteinyl residues, which are bound to zinc, do not react. However, they do react in the presence of a chelating agent such as EDTA. The resultant difference in chemical reactivity provides a means to measure T in the absence of EDTA, (MT + T) in its presence, and, of course, MT by difference. The 7-fluorobenz-2-oxa-1,3-diazole-4-sulfonamide derivative of T can be isolated from tissue homogenates by HPLC and quantified fluorimetrically with a detection limit in the femtomolar range and a linear response over 3 orders of magnitude. Analysis of liver, kidney, and brain of rats reveals almost as much T as MT. Moreover, in contrast to earlier views, MT in tissue extracts appears to be less stable than T. The existence of T in tissues under normal physiological conditions has important implications for its function both in zinc metabolism and the redox balance of the cell.
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