ArticleProceedings of the National Academy of Sciences of the United States of America2001
Trans-spliced leader addition to mRNAs in a cnidarian.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 116 citations in OpenAlex.
- The dynamic genomes of Hydra and the anciently active repeat complement of animal chromosomes.Genome biology · 2025Article
- Molecular cytogenetic study on the scleractinian coralComparative cytogenetics · 2025Article
- The genome of the colonial hydroidGenome research · 2024Article
- Extreme genome scrambling in marine planktonicGenome research · 2024Article
- Genetic interference with HvNotch provides new insights into the role of the Notch-signalling pathway for developmental pattern formation in Hydra.Scientific reports · 2024Article
- A diversified and segregated mRNA spliced-leader system in the parasitic Perkinsozoa.Open biology · 2022Article
- Karyotypic analysis and isolation of four DNA markers of the scleractinian coralComparative cytogenetics · 2022Article
- Differential gene regulation in DAPT-treated Hydra reveals candidate direct Notch signalling targets.Journal of cell science · 2021Article
- Cytogenetic markers using single-sequence probes reveal chromosomal locations of tandemly repetitive genes in scleractinian coral Acropora pruinosa.Scientific reports · 2021Article
- Screening a Spliced Leader-BasedMicroorganisms · 2021Article
- SLIDR and SLOPPR: flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data.BMC bioinformatics · 2021Article
- SLFinder, a pipeline for the novel identification of splice-leader sequences: a good enough solution for a complex problem.BMC bioinformatics · 2020Article
- Stem cell differentiation trajectories inScience (New York, N.Y.) · 2019Article
- Article
- Heterodera glycines utilizes promiscuous spliced leaders and demonstrates a unique preference for a species-specific spliced leader over C. elegans SL1.Scientific reports · 2019Article
- The genome of the jellyfish Aurelia and the evolution of animal complexity.Nature ecology & evolution · 2019Article
- Article
- On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.Journal of molecular evolution · 2017Review
- Co-evolution of SNF spliceosomal proteins with their RNA targets in trans-splicing nematodes.Genetica · 2016Article
- Evolutionary Insights into RNA trans-Splicing in Vertebrates.Genome biology and evolution · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A search of databases with the sequence from the 5' untranslated region of a Hydra cDNA clone encoding a receptor protein-tyrosine kinase revealed that a number of Hydra cDNAs contain one of two different sequences at their 5' ends. This finding suggested the possibility that mRNAs in Hydra receive leader sequences by trans-splicing. This hypothesis was confirmed by the finding that the leader sequences are transcribed as parts of small RNAs encoded by genes located in the 5S rRNA clusters of Hydra. The two spliced leader (SL) RNAs (SL-A and -B) contain splice donor dinucleotides at the predicted positions, and genes that receive SLs contain splice acceptor dinucleotides at the predicted positions. Both of the SL RNAs are bound by antibody against trimethylguanosine, suggesting that they contain a trimethylguanosine cap. The predicted secondary structures of the Hydra SL RNAs show significant differences from the structures predicted for the SLs of other organisms. Messenger RNAs have been identified that can receive either SL-A or -B, although the impact of the two different SLs on the function of the mRNA is unknown. The presence and features of SL addition in the phylum Cnidaria raise interesting questions regarding the evolution of this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.