ArticleProceedings of the National Academy of Sciences of the United States of America2001
Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 77 citations in OpenAlex.
- Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation.Nucleic acids research · 2026Article
- The unique kind of human artificial chromosome: Bypassing the requirement for repetitive centromere DNA.Experimental cell research · 2020Review
- Article
- De novo genome assembly ofCommunications biology · 2018Article
- Genetic and epigenetic regulation of centromeres: a look at HAC formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Circular permutation of a synthetic eukaryotic chromosome with the telomerator.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Repeatless and repeat-based centromeres in potato: implications for centromere evolution.The Plant cell · 2012Article
- Human centromere genomics: now it's personal.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Active transcription and essential role of RNA polymerase II at the centromere during mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Human artificial chromosomes for gene delivery and the development of animal models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Towards the development of better crops by genetic transformation using engineered plant chromosomes.Plant cell reports · 2011Review
- Artificial chromosome formation in maize (Zea mays L.).Chromosoma · 2009Article
- Neocentromeres: new insights into centromere structure, disease development, and karyotype evolution.American journal of human genetics · 2008Review
- A minimal CENP-A core is required for nucleation and maintenance of a functional human centromere.The EMBO journal · 2007Article
- Telomere-mediated chromosomal truncation in maize.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Mouse telocentric sequences reveal a high rate of homogenization and possible role in Robertsonian translocation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Artificial and engineered chromosomes: developments and prospects for gene therapy.Chromosoma · 2005Review
- The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer.Chromosoma · 2005Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neocentromeres (NCs) are fully functional centromeres that arise ectopically in noncentromeric regions lacking alpha-satellite DNA. Using telomere-associated chromosome truncation, we have produced a series of minichromosomes (MiCs) from a mardel(10) marker chromosome containing a previously characterized human NC. These MiCs range in size from approximately 0.7 to 1.8 Mb and contain single-copy intact genomic DNA from the 10q25 region. Two of these NC-based Mi-Cs (NC-MiCs) appear circular whereas one is linear. All demonstrate stability in both structure and mitotic transmission in the absence of drug selection. Presence of a functional NC is shown by binding a host of key centromere-associated proteins. These NC-MiCs provide direct evidence for mitotic segregation function of the NC DNA and represent examples of stable mammalian MiCs lacking centromeric repeats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.