ArticleProceedings of the National Academy of Sciences of the United States of America2001
Proteomic dissection of dome formation in a mammary cell line: role of tropomyosin-5b and maspin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 32 citations in OpenAlex.
- Transmembrane Protein TMEM230, Regulator of Glial Cell Vascular Mimicry and Endothelial Cell Angiogenesis in High-Grade Heterogeneous Infiltrating Gliomas and Glioblastoma.International journal of molecular sciences · 2024Article
- Effect of luminal flow on doming of mpkCCD cells in a 3D perfusable kidney cortical collecting duct model.American journal of physiology. Cell physiology · 2020Article
- Actin-tropomyosin distribution in non-muscle cells.Journal of muscle research and cell motility · 2020Review
- Aquaporin 5 expression in mouse mammary gland cells is not driven by promoter methylation.BioMed research international · 2015Article
- Characterization of the osteoblast-specific transmembrane protein IFITM5 and analysis of IFITM5-deficient mice.Journal of bone and mineral metabolism · 2011Article
- Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The properties of a mammary gland cancer stem cell.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes.Genes and immunity · 2006Article
- Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Proteomic dissection of dome formation in a mammary cell line.Journal of mammary gland biology and neoplasia · 2002Review
- Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work we extended the study of genes controlling the formation of specific differentiation structures called "domes" formed by the rat mammary adenocarcinoma cell line LA7 under the influence of DMSO. We have reported previously that an interferon-inducible gene, rat-8, and the beta-subunit of the epithelial sodium channel (ENaC) play a fundamental role in this process. Now, we used a proteomic approach to identify proteins differentially expressed either in DMSO-induced LA7 or in 106A10 cells. Two differentially expressed proteins were investigated. The first, tropomyosin-5b, strongly expressed in DMSO-induced LA7 cells, is needed for dome formation because its synthesis inhibition by the antisense RNA technology abolished domes. The second protein, maspin, strongly expressed in the uninduced 106A10 cell line, inhibits dome formation because 106A10 cells, transfected with rat8 cDNA (the function of which is required for the organization of these structures), acquired the ability to develop domes when cultured in presence of an antimaspin antibody. Dome formation in these cultures are accompanied by ENaC beta-subunit expression in the absence of DMSO. Therefore, dome formation requires the expression of tropomyosin-5b, in addition to the ENaC beta-subunit and the rat8 proteins, and is under the negative control of maspin.
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