ArticleThe EMBO journal2001
SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Arginine methylation-dependent METTL14-SMN interaction regulates RNA mEMBO reports · 2025Article
- The role of Arabidopsis Splicing Factor 30 in floral transition and the implications of its alternative splicing.Plant physiology · 2025Article
- Article
- An autoregulatory poison exon in Smndc1 is conserved across kingdoms and influences organism growth.PLoS genetics · 2024Article
- Pharmacological perturbation of the phase-separating protein SMNDC1.Nature communications · 2023Article
- exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers.Cell genomics · 2023Article
- Effectors and effects of arginine methylation.Biochemical Society transactions · 2023Article
- PICLN modulates alternative splicing and light/temperature responses in plants.Plant physiology · 2023Article
- The SMN Complex at the Crossroad between RNA Metabolism and Neurodegeneration.International journal of molecular sciences · 2023Review
- Cell-cycle and apoptosis related and proteomics-based signaling pathways of human hepatoma Huh-7 cells treated by three currently used multi-RTK inhibitors.Frontiers in pharmacology · 2022Article
- Article
- A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing.PLoS genetics · 2019Article
- Fragile X mental retardation protein recognizes a G quadruplex structure within the survival motor neuron domain containing 1 mRNA 5'-UTR.Molecular bioSystems · 2017Article
- Article
- Proteomic profile of embryonic stem cells with low survival motor neuron protein is consistent with developmental dysfunction.Journal of neural transmission (Vienna, Austria : 1996) · 2017Article
- A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.Nature communications · 2016Article
- Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice site.RNA (New York, N.Y.) · 2015Article
- Impaired Muscle Mitochondrial Biogenesis and Myogenesis in Spinal Muscular Atrophy.JAMA neurology · 2015Article
- Readers of histone methylarginine marks.Biochimica et biophysica acta · 2014Review
- Behavioral and electrophysiological outcomes of tissue-specific Smn knockdown in Drosophila melanogaster.Brain research · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SMNrp, also termed SPF30, has recently been identified in spliceosomes assembled in vitro. We have functionally characterized this protein and show that it is an essential splicing factor. We show that SMNrp is a 17S U2 snRNP-associated protein that appears in the pre-spliceosome (complex A) and the mature spliceosome (complex B) during splicing. Immunodepletion of SMNrp from nuclear extract inhibits the first step of pre-mRNA splicing by preventing the formation of complex B. Re-addition of recombinant SMNrp to immunodepleted extract reconstitutes both spliceosome formation and splicing. Mutations in two domains of SMNrp, although similarly deleterious for splicing, differed in their consequences on U2 snRNP binding, suggesting that SMNrp may also engage in interactions with splicing factors other than the U2 snRNP. In agreement with this, we present evidence for an additional interaction between SMNrp and the [U4/U6.U5] tri-snRNP. A candidate that may mediate this interaction, namely the U4/U6-90 kDa protein, has been identified. We suggest that SMNrp, as a U2 snRNP-associated protein, facilitates the recruitment of the [U4/U6.U5] tri-snRNP to the pre-spliceosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.