ArticleThe Journal of cell biology2001
Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed, 154 citations in OpenAlex.
- Keratin filaments and soluble vimentin interact with isoform specificity to organize cell signaling.Genes & development · 2026Article
- A transitional desmosome/tonofibril network may relay mechanical strain to epidermal nerve terminals with high fidelity and sensitivity in the Cuban crocodile (Frontiers in cell and developmental biology · 2026Article
- SRC and ERK Regulate the Turnover of Cytoskeletal Keratin Filaments.International journal of molecular sciences · 2025Article
- Tandem LIM domain-containing proteins, LIMK1 and LMO1, directly bind to force-bearing keratin intermediate filaments.Cell reports · 2024Article
- Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells.Communications biology · 2023Article
- Bend, Push, Stretch: Remarkable Structure and Mechanics of Single Intermediate Filaments and Meshworks.Cells · 2021Review
- Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy.eLife · 2021Article
- Hemidesmosome-Related Keratin Filament Bundling and Nucleation.International journal of molecular sciences · 2021Article
- M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs.Journal of materials science. Materials in medicine · 2020Article
- Article
- Keratin Dynamics and Spatial Distribution in Wild-Type and K14 R125P Mutant Cells-A Computational Model.International journal of molecular sciences · 2020Article
- The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis.Cellular and molecular life sciences : CMLS · 2020Article
- PAK2 links cell survival to mechanotransduction and metabolism.The Journal of cell biology · 2019Article
- Review
- Protein Interaction Analysis Provides a Map of the Spatial and Temporal Organization of the Ciliary Gating Zone.Current biology : CB · 2017Article
- Intracellular Motility of Intermediate Filaments.Cold Spring Harbor perspectives in biology · 2017Review
- Intermediate Filaments at the Junction of Mechanotransduction, Migration, and Development.Frontiers in cell and developmental biology · 2017Review
- GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions.Cellular and molecular life sciences : CMLS · 2016Article
- Intermediate filament dynamics: What we can see now and why it matters.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Vimentin filament precursors exchange subunits in an ATP-dependent manner.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The properties of keratin intermediate filaments (IFs) have been studied after transfection with green fluorescent protein (GFP)-tagged K18 and/or K8 (type I/II IF proteins). GFP-K8 and -K18 become incorporated into tonofibrils, which are comprised of bundles of keratin IFs. These tonofibrils exhibit a remarkably wide range of motile and dynamic activities. Fluorescence recovery after photobleaching (FRAP) analyses show that they recover their fluorescence slowly with a recovery t(1/2) of approximately 100 min. The movements of bleach zones during recovery show that closely spaced tonofibrils (<1 microm apart) often move at different rates and in different directions. Individual tonofibrils frequently change their shapes, and in some cases these changes appear as propagated waveforms along their long axes. In addition, short fibrils, termed keratin squiggles, are seen at the cell periphery where they move mainly towards the cell center. The motile properties of keratin IFs are also compared with those of type III IFs (vimentin) in PtK2 cells. Intriguingly, the dynamic properties of keratin tonofibrils and squiggles are dramatically different from those of vimentin fibrils and squiggles within the same cytoplasmic regions. This suggests that there are different factors regulating the dynamic properties of different types of IFs within the same cytoplasmic regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.