ArticleThe Journal of cell biology2001
Inhibitory role of alpha 6 beta 4-associated erbB-2 and phosphoinositide 3-kinase in keratinocyte haptotactic migration dependent on alpha 3 beta 1 integrin.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 108 citations in OpenAlex.
- Article
- Polymeric immunoglobulin receptor suppresses colorectal cancer through the AKT-FOXO3/4 axis by downregulating LAMB3 expression.Frontiers in oncology · 2022Article
- Feeling Things Out: Bidirectional Signaling of the Cell-ECM Interface, Implications in the Mechanobiology of Cell Spreading, Migration, Proliferation, and Differentiation.Advanced healthcare materials · 2020Review
- Article
- Targeting β1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice.Nature medicine · 2016Article
- Circulatory shear flow alters the viability and proliferation of circulating colon cancer cells.Scientific reports · 2016Article
- Promotion of cell migration by neural cell adhesion molecule (NCAM) is enhanced by PSA in a polysialyltransferase-specific manner.PloS one · 2015Article
- The metastasis-associated molecule C4.4A promotes tissue invasion and anchorage independence by associating with the alpha6beta4 integrin.Molecular oncology · 2013Article
- The role of integrins in the development and homeostasis of the epidermis and skin appendages.Acta naturae · 2013Article
- Collagen VII plays a dual role in wound healing.The Journal of clinical investigation · 2013Article
- Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.Molecular biology of the cell · 2012Article
- Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis.The Journal of biological chemistry · 2011Article
- Article
- Transcriptome analysis of human cancer reveals a functional role of heme oxygenase-1 in tumor cell adhesion.Molecular cancer · 2010Article
- Transdominant regulation of integrin function: mechanisms of crosstalk.Cellular signalling · 2010Review
- Disruption of laminin-integrin-CD151-focal adhesion kinase axis sensitizes breast cancer cells to ErbB2 antagonists.Cancer research · 2010Article
- Laminin-binding integrins and their tetraspanin partners as potential antimetastatic targets.Expert reviews in molecular medicine · 2010Review
- Emerging concepts in engineering extracellular matrix variants for directing cell phenotype.Regenerative medicine · 2009Review
- BPAG1e maintains keratinocyte polarity through beta4 integrin-mediated modulation of Rac1 and cofilin activities.Molecular biology of the cell · 2009Article
- Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing.PloS one · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Keratinocytes and other epithelial cells express two receptors for the basement membrane (BM) extracellular matrix component laminin-5 (Ln-5), integrins alpha 3 beta 1 and alpha 6 beta 4. While alpha 3 beta 1 mediates adhesion, spreading, and migration (Kreidberg, J.A. 2000. Curr. Opin. Cell Biol. 12:548--553), alpha 6 beta 4 is involved in BM anchorage via hemidesmosomes (Borradori, L., and A. Sonnenberg. 1999. J. Invest. Dermatol. 112:411--418). We investigated a possible regulatory interplay between alpha 3 beta 1 and alpha 6 beta 4 in cell motility using HaCaT keratinocytes as a model. We found that alpha 6 beta 4 antibodies inhibit alpha 3 beta 1-mediated migration on Ln-5, but only when migration is haptotactic (i.e., spontaneous or stimulated by alpha 3 beta 1 activation), and not when chemotactic (i.e., triggered by epidermal growth factor receptor). Inhibition of migration by alpha 6 beta 4 depends upon phosphoinositide 3-kinase (PI3-K) since it is abolished by PI3-K blockers and by dominant-negative PI3-K, and constitutively active PI3-K prevents haptotaxis. In HaCaT cells incubated with anti-alpha 6 beta 4 antibodies, activation of PI3-K is mediated by alpha 6 beta 4-associated erbB-2, as indicated by erbB-2 autophosphorylation and erbB-2/p85 PI3-K coprecipitation. Furthermore, dominant-negative erbB-2 abolishes inhibition of haptotaxis by anti-alpha 6 beta 4 antibodies. These results support a model whereby (a) haptotactic cell migration on Ln-5 is regulated by concerted action of alpha 3beta 1 and alpha 6 beta 4 integrins, (b) alpha 6 beta 4-associated erbB-2 and PI3-K negatively affect haptotaxis, and (c) chemotaxis on Ln-5 is not affected by alpha 6 beta 4 antibodies and may require PI3-K activity. This model could be of general relevance to motility of epithelial cells in contact with BM.
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