ArticleClinical and diagnostic laboratory immunology2001
Western immunoblotting with five Treponema pallidum recombinant antigens for serologic diagnosis of syphilis.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
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- Development of a Bead-Based Multiplex Assay for Use in Multianalyte Screening and Surveillance of HIV, Viral Hepatitis, Syphilis, and Herpes.Journal of clinical microbiology · 2022Article
- Comparison of "Lumipulse anti-Treponema pallidum" and "Architect Syphilis TP" and further examination.Journal of clinical laboratory analysis · 2020Article
- Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis.PloS one · 2020Article
- Syphilis.Nature reviews. Disease primers · 2017Review
- NovelBioMed research international · 2017Review
- Contribution of a Comparative Western Blot Method to Early Postnatal Diagnosis of Congenital Syphilis.Clinical and vaccine immunology : CVI · 2016Article
- Evaluation of the HISCL Anti-Treponema pallidum Assay as a Screening Test for Syphilis.Clinical and vaccine immunology : CVI · 2015Article
- Recent trends in the serologic diagnosis of syphilis.Clinical and vaccine immunology : CVI · 2015Review
- Performance evaluation of the Elecsys syphilis assay for the detection of total antibodies to Treponema pallidum.Clinical and vaccine immunology : CVI · 2015Article
- Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis.Clinical and vaccine immunology : CVI · 2013Article
- Evaluation of the BioPlex 2200 syphilis system as a first-line method of reverse-sequence screening for syphilis diagnosis.Clinical and vaccine immunology : CVI · 2013Article
- Humans parasitized by the hard tick Ixodes ricinus are seropositive to Midichloria mitochondrii: is Midichloria a novel pathogen, or just a marker of tick bite?Pathogens and global health · 2012Article
- The immunopathobiology of syphilis: the manifestations and course of syphilis are determined by the level of delayed-type hypersensitivity.The American Journal of dermatopathology · 2011Review
- Congenital syphilis like many years ago.Case reports in infectious diseases · 2011Article
- The potential of backscattering interferometry as an in vitro clinical diagnostic tool for the serological diagnosis of infectious disease.The Analyst · 2010Article
- Chlamydia trachomatis serovar distribution and other concurrent sexually transmitted infections in heterosexual men with urethritis in Italy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Laboratory diagnosis of syphilis with automated immunoassays.Journal of clinical laboratory analysis · 2009Article
- Prenatal syphilis infection is a possible cause of preterm delivery among immigrant women from eastern Europe.Sexually transmitted infections · 2007Article
- Production of reactive oxygen species and expression of inducible nitric oxide synthase in rat isolated Kupffer cells stimulated by Leptospira interrogans and Borrelia burgdorferi.World journal of gastroenterology · 2006Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.
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