ArticleClinical and diagnostic laboratory immunology2001
Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- Molecular epidemiology of group A rotavirus (RVA) and bovine coronavirus (BCoV) associated with neonatal calf diarrhea in Türkiye.Tropical animal health and production · 2026Article
- Development and evaluation of an indirect ELISA for detecting ovine rotavirus antibodies based on VP6 protein.BMC veterinary research · 2026Article
- Diagnosis of bovine rotavirus: an overview of currently available methods.Frontiers in microbiology · 2025Review
- Determining genetic diversity of prevalent G and P genotype ofJournal of veterinary science · 2024Article
- Establishment of an Indirect ELISA Method for the Detection of the Bovine Rotavirus VP6 Protein.Animals : an open access journal from MDPI · 2024Article
- Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan.BMC gastroenterology · 2019Article
- Rapid detection of human rotavirus using NSP4 gene specific reverse transcription loop-mediated isothermal amplification assay.Indian journal of virology : an official organ of Indian Virological Society · 2013Article
- Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection.Microbial biotechnology · 2013Article
- Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia.Australian veterinary journal · 2012Article
- Viral and bacterial infections associated with camel (Camelus dromedarius) calf diarrhea in North Province, Saudi Arabia.Saudi journal of biological sciences · 2012Article
- Development of a latex agglutination test for norovirus detection.Journal of microbiology (Seoul, Korea) · 2010Article
- Genotype 1 and genotype 2 bovine noroviruses are antigenically distinct but share a cross-reactive epitope with human noroviruses.Journal of clinical microbiology · 2006Article
- Verification of sensitivity and specificity of group a rotavirus detection in piglets faeces with monoclonal blocking ELISA methods.Journal of veterinary medicine. B, Infectious diseases and veterinary public health · 2004Article
- Evaluation of a human group a rotavirus assay for on-site detection of bovine rotavirus.Journal of clinical microbiology · 2003Article
- Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples.Clinical and diagnostic laboratory immunology · 2002Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect the presence of group A rotavirus antigen. Of the 63 samples screened by VI-FA, 33 (58%) tested positive for rotavirus antigen. When the results from the LAT were compared to those from VI-FA, the "gold standard" for detection of bovine rotavirus in fecal samples, the sensitivity and specificity were found to be 87.8 and 73.3%, respectively. Latex agglutination compared with ELISA (the reference method) showed 100% sensitivity and 96.3% specificity, and when ELISA was compared with VI, the sensitivity was 84.8% and the specificity was 73.3%. Latex agglutination is easy to perform in a short time and does not require expensive equipment or skilled personnel, and the reagents have long shelf lives. These factors make the LAT suitable and highly efficient for use in a clinical laboratory as a rapid screening test for bovine rotavirus.
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