ArticleJournal of clinical pathology2001
Rapid identification of candida species by TaqMan PCR.
Article in Journal of clinical pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Empirical voriconazole therapy for febrile neutropenic patients with hematological disorders: a prospective multicenter trial in Japan.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2013Trial
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- Candida species-specific colonization in the healthy and impaired human gastrointestinal tract as simulated using the Mucosal Ileum-SHIME® model.FEMS microbiology ecology · 2024Article
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- UnveilingGut microbes · 2023Article
- Improved RAPD Method forGenes · 2023Article
- Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption.AMB Express · 2023Article
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- Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics.Frontiers in cellular and infection microbiology · 2022Article
- Associations of the oral microbiota and Candida with taste, smell, appetite and undernutrition in older adults.Scientific reports · 2021Article
- Generation and Characterization of Single Chain Variable Fragment against Alpha-Enolase ofInternational journal of molecular sciences · 2020Article
- A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions.Scientific reports · 2019Article
- Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.Journal of clinical microbiology · 2016Article
- Rapid Detection of Candida albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples.Frontiers in microbiology · 2016Article
- Article
- Multi-probe real-time PCR identification of four common Candida species in blood culture broth.Mycopathologia · 2014Article
- Oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2012Article
- Identification and differential gene expression of adhesin-like wall proteins in Candida glabrata biofilms.Mycopathologia · 2011Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop and evaluate a TaqMan(TM) polymerase chain reaction (PCR) for the rapid identification and speciation of candida species.
methodsSpecies specific primer and probe sets were designed for the identification of Candida albicans, C. parapsilosis, C. tropicalis, C. krusei, C. kefyr, and C. glabrata from clinical isolates in a 5' exonuclease (TaqMan(TM)) assay. The probes were labelled with three fluorescent dyes to enable differentiation between species when three primer and probe sets were combined in two multiplexes. The specificity of these assays was evaluated against a range of National Collection of Pathogenic Fungi strains, clinical isolates of yeast, bacterial and viral pathogens.
resultsThe primer and probe sets have been shown to be 100% specific for their respective species; there was no crossreaction between any set and human DNA, or extracts from other candida species, fungal, bacterial, or viral pathogens tested. Extracts from two clinical isolates, originally identified as C albicans on the basis of germ tube formation, were not amplified by any of the primer and probe sets. These isolates have been putatively re-identified as C dubliniensis after sequencing of the variable internal transcribed spacer region ITS2 and lack of growth at 45 degrees C.
conclusionThis TaqMan assay provides a rapid alternative to conventional culture based techniques for the identification and speciation of the most frequently isolated candida species. The simple extraction method followed by TaqMan PCR can identify the six species mentioned in four hours.
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