ArticleJournal of bacteriology2001
Tagging morphogenetic genes by insertional mutagenesis in the yeast Yarrowia lipolytica.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- High-throughput metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mar1, a high mobility group box protein, regulatesApplied and environmental microbiology · 2024Article
- FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides.Applied microbiology and biotechnology · 2024Article
- Genetic inactivation of the Carnitine/Acetyl-Carnitine mitochondrial carrier of Yarrowia lipolytica leads to enhanced odd-chain fatty acid production.Microbial cell factories · 2023Article
- Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Copper Ion Mediates Yeast-to-Hypha Transition inJournal of fungi (Basel, Switzerland) · 2023Article
- The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis.Plants (Basel, Switzerland) · 2019Review
- New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.Microbial cell factories · 2017Article
- Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production.Journal of visualized experiments : JoVE · 2016Article
- Kexin-like endoprotease KexB is required for N-glycan processing, morphogenesis and virulence in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2015Article
- A role for the rap GTPase YlRsr1 in cellular morphogenesis and the involvement of YlRsr1 and the ras GTPase YlRas2 in bud site selection in the dimorphic yeast Yarrowia lipolytica.Eukaryotic cell · 2014Article
- Zinc finger transcription factors displaced SREBP proteins as the major Sterol regulators during Saccharomycotina evolution.PLoS genetics · 2014Article
- Lack of the catalytic subunit of telomerase leads to growth defects accompanied by structural changes at the chromosomal ends in Yarrowia lipolytica.Current genetics · 2010Article
- The gluconeogenic enzyme fructose-1,6-bisphosphatase is dispensable for growth of the yeast Yarrowia lipolytica in gluconeogenic substrates.Eukaryotic cell · 2008Article
- Yarrowia lipolytica mutants devoid of pyruvate carboxylase activity show an unusual growth phenotype.Eukaryotic cell · 2005Article
- Genetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica.Genetics · 2002Article
- Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization.Journal of bacteriology · 2001Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The yeast Yarrowia lipolytica is distantly related to Saccharomyces cerevisiae, can be genetically modified, and can grow in both haploid and diploid states in either yeast, pseudomycelial, or mycelial forms, depending on environmental conditions. Previous results have indicated that the STE and RIM pathways, which mediate cellular switching in other dimorphic yeasts, are not required for Y. lipolytica morphogenesis. To identify the pathways involved in morphogenesis, we mutagenized a wild-type strain of Y. lipolytica with a Tn3 derivative. We isolated eight tagged mutants, entirely defective in hyphal formation, from a total of 40,000 mutants and identified seven genes homologous to S. cerevisiae CDC25, RAS2, BUD6, KEX2, GPI7, SNF5, and PPH21. We analyzed their abilities to invade agar and to form pseudomycelium or hyphae under inducing conditions and their sensitivity to temperature and to Calcofluor white. Chitin staining was used to detect defects in their cell walls. Our results indicate that a functional Ras-cyclic AMP pathway is required for the formation of hyphae in Y. lipolytica and that perturbations in the processing of extracellular, possibly parietal, proteins result in morphogenetic defects.
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