ArticleJournal of bacteriology2001
Role of Escherichia coli nitrogen regulatory genes in the nitrogen response of the Azotobacter vinelandii NifL-NifA complex.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Molecular Mechanism and Agricultural Application of the NifA-NifL System for Nitrogen Fixation.International journal of molecular sciences · 2023Review
- Molecular Origins of Transcriptional Heterogeneity in Diazotrophic Klebsiella oxytoca.mSystems · 2022Article
- Enhanced production of polyhydroxyalkanoate with manipulable and reproducible 3-hydroxyvalerate fraction by high alcohol tolerant Cupriavidus malaysiensis USMAA2-4 transformant.Bioprocess and biosystems engineering · 2022Article
- Disrupting hierarchical control of nitrogen fixation enables carbon-dependent regulation of ammonia excretion in soil diazotrophs.PLoS genetics · 2021Article
- Imbalance in Carbon and Nitrogen Metabolism in Comamonas testosteroni R2 Is Caused by Negative Feedback and Rescued by L-arginine.Microbes and environments · 2021Article
- Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria.Life (Basel, Switzerland) · 2018Article
- Transcriptional Control of Dual Transporters Involved in α-Ketoglutarate Utilization Reveals Their Distinct Roles in UropathogenicFrontiers in microbiology · 2017Article
- The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.Microbiology and molecular biology reviews : MMBR · 2015Review
- The trpE gene negatively regulates differentiation of heterocysts at the level of induction in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2015Article
- Influence of PAS domain flanking regions on oligomerisation and redox signalling by NifL.PloS one · 2012Article
- Insights into membrane association of Klebsiella pneumoniae NifL under nitrogen-fixing conditions from mutational analysis.Journal of bacteriology · 2011Article
- Control of AmtB-GlnK complex formation by intracellular levels of ATP, ADP, and 2-oxoglutarate.The Journal of biological chemistry · 2010Article
- Identification and functional characterization of NifA variants that are independent of GlnB activation in the photosynthetic bacterium Rhodospirillum rubrum.Microbiology (Reading, England) · 2008Article
- Comparative genomic analyses of the bacterial phosphotransferase system.Microbiology and molecular biology reviews : MMBR · 2005Review
- Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A crucial arginine residue is required for a conformational switch in NifL to regulate nitrogen fixation in Azotobacter vinelandii.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The NifL-NifA System: a multidomain transcriptional regulatory complex that integrates environmental signals.Journal of bacteriology · 2004Review
- Domain architectures of sigma54-dependent transcriptional activators.Journal of bacteriology · 2003Review
- Mutant forms of the Azotobacter vinelandii transcriptional activator NifA resistant to inhibition by the NifL regulatory protein.Journal of bacteriology · 2002Article
- Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB.The EMBO journal · 2002Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
The redox-sensing flavoprotein NifL inhibits the activity of the nitrogen fixation (nif)-specific transcriptional activator NifA in Azotobacter vinelandii in response to molecular oxygen and fixed nitrogen. Although the mechanism whereby the A. vinelandii NifL-NifA system responds to fixed nitrogen in vivo is unknown, the glnK gene, which encodes a PII-like signal transduction protein, has been implicated in nitrogen control. However, the precise function of A. vinelandii glnK in this response is difficult to establish because of the essential nature of this gene. We have shown previously that A. vinelandii NifL is able to respond to fixed nitrogen to control NifA activity when expressed in Escherichia coli. In this study, we investigated the role of the E. coli PII-like signal transduction proteins in nitrogen control of the A. vinelandii NifL-NifA regulatory system in vivo. In contrast to recent findings with Klebsiella pneumoniae NifL, our results indicate that neither the E. coli PII nor GlnK protein is required to relieve inhibition by A. vinelandii NifL under nitrogen-limiting conditions. Moreover, disruption of both the E. coli glnB and ntrC genes resulted in a complete loss of nitrogen regulation of NifA activity by NifL. We observe that glnB ntrC and glnB glnK ntrC mutant strains accumulate high levels of intracellular 2-oxoglutarate under conditions of nitrogen excess. These findings are in accord with our recent in vitro observations (R. Little, F. Reyes-Ramirez, Y. Zhang, W. Van Heeswijk, and R. Dixon, EMBO J. 19:6041-6050, 2000) and suggest a model in which nitrogen control of the A. vinelandii NifL-NifA system is achieved through the response to the level of 2-oxoglutarate and an interaction with PII-like proteins under conditions of nitrogen excess.
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