ArticleBiophysical journal2001
Hydration state of single cytochrome c monolayers on soft interfaces via neutron interferometry.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Voltage-Dependent Profile Structures of a Kv-Channel via Time-Resolved Neutron Interferometry.Biophysical journal · 2019Article
- Computational de novo design and characterization of a protein that selectively binds a highly hyperpolarizable abiological chromophore.Journal of the American Chemical Society · 2013Article
- Structural characterization of the voltage-sensor domain and voltage-gated K+-channel proteins vectorially oriented within a single bilayer membrane at the solid/vapor and solid/liquid interfaces via neutron interferometry.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Profile structures of the voltage-sensor domain and the voltage-gated K(+)-channel vectorially oriented in a single phospholipid bilayer membrane at the solid-vapor and solid-liquid interfaces determined by x-ray interferometry.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Molecular dynamics simulations of a hydrated protein vectorially oriented on polar and nonpolar soft surfaces.Biophysical journal · 2002Article
- Orientation distributions for cytochrome c on polar and nonpolar interfaces by total internal reflection fluorescence.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast cytochrome c (YCC) can be covalently tethered to, and thereby vectorially oriented on, the soft surface of a mixed endgroup (e.g., -CH3/-SH = 6:1, or -OH/-SH = 6:1) organic self-assembled monolayer (SAM) chemisorbed on the surface of a silicon substrate utilizing a disulfide linkage between its unique surface cysteine residue and a thiol endgroup. Neutron reflectivities from such monolayers of YCC on Fe/Si or Fe/Au/Si multilayer substrates with H2O versus D2O hydrating the protein monolayer at 88% relative humidity for the nonpolar SAM (-CH3/-SH = 6:1 mixed endgroups) surface and 81% for the uncharged-polar SAM (-OH/-SH = 6:1mixed endgroups) surface were collected on the NG1 reflectometer at NIST. These data were analyzed using a new interferometric phasing method employing the neutron scattering contrast between the Si and Fe layers in a single reference multilayer structure and a constrained refinement approach utilizing the finite extent of the gradient of the profile structures for the systems. This provided the water distribution profiles for the two tethered protein monolayers consistent with their electron density profile determined previously via x-ray interferometry (Chupa et al., 1994).
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