ArticleBiophysical journal2001
Actin modifies Ca2+ block of epithelial Na+ channels in planar lipid bilayers.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Physical and functional interactions between a glioma cation channel and integrin-β1 require α-actinin.American journal of physiology. Cell physiology · 2015Article
- The plasma membrane potential and the organization of the actin cytoskeleton of epithelial cells.International journal of cell biology · 2012Article
- Regulation of epithelial sodium transport via epithelial Na+ channel.Journal of biomedicine & biotechnology · 2011Review
- Regulation of the epithelial Na+ channel and airway surface liquid volume by serine proteases.Pflugers Archiv : European journal of physiology · 2010Review
- Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel.PloS one · 2010Article
- Decoding epithelial signals: critical role for the epidermal growth factor receptor in controlling intestinal transport function.Acta physiologica (Oxford, England) · 2009Review
- Liquid movement across the surface epithelium of large airways.Respiratory physiology & neurobiology · 2007Review
- Regulation of the epithelial sodium channel by phosphatidylinositides: experiments, implications, and speculations.Pflugers Archiv : European journal of physiology · 2007Review
- Actin and amphiphilic polymers influence on channel formation by Syringomycin E in lipid bilayers.European biophysics journal : EBJ · 2006Article
- Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.The Journal of general physiology · 2005Article
- UTP-dependent inhibition of Na+ absorption requires activation of PKC in endometrial epithelial cells.The Journal of general physiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+]free was buffered to <10 nM. An analysis of single-channel kinetics demonstrated that Ca2+ induced the appearance of long-lived closed intervals separating bursts of channel activity, both in the presence and in the absence of actin. In the absence of actin, the duration of these bursts and the time spent by the channel in its open, but not in its short-lived closed state, were inversely proportional to [Ca2+]. This, together with a lengthening of the interburst intervals, translated into a dose-dependent decrease in the single-channel open probability. In contrast, a [Ca2+]-dependent decrease in alpha-ENaC conductance in the presence of actin was accompanied by lengthening of the burst intervals with no significant changes in the open or closed (both short- and long-lived) times. We conclude that Ca2+ acts as a "fast-to-intermediate" blocker when monomeric actin is present, producing a subsequent attenuation of the apparent unitary conductance of the channel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.