ArticleBiophysical journal2001
Mouse mast cell secretory granules can function as intracellular ionic oscillators.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Article
- CRISPR/Cas9-mediated endogenous C-terminal Tagging of Trypanosoma cruzi Genes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor.The Journal of biological chemistry · 2016Article
- Acidocalcisomes of Trypanosoma brucei have an inositol 1,4,5-trisphosphate receptor that is required for growth and infectivity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Orai-STIM-mediated Ca2+ release from secretory granules revealed by a targeted Ca2+ and pH probe.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Time-resolved release of calcium from an epithelial cell monolayer during mucin secretion.European biophysics journal : EBJ · 2011Article
- Ca2+ dynamics in the secretory vesicles of neurosecretory PC12 and INS1 cells.Cellular and molecular neurobiology · 2010Article
- Inositol 1,4,5-trisphosphate receptor in chromaffin secretory granules and its relation to chromogranins.Cellular and molecular neurobiology · 2010Review
- Secretory granules in inositol 1,4,5-trisphosphate-dependent Ca2+ signaling in the cytoplasm of neuroendocrine cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Review
- Review
- SK channels mediate NADPH oxidase-independent reactive oxygen species production and apoptosis in granulocytes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- InsP3 signaling induces pulse-modulated Ca2+ signals in the nucleus of airway epithelial ciliated cells.Biophysical journal · 2005Article
- Ryanodine receptors in peritoneal mast cells: possible role in the modulation of exocytotic activity.Pflugers Archiv : European journal of physiology · 2004Article
- ATP-independent luminal oscillations and release of Ca2+ and H+ from mast cell secretory granules: implications for signal transduction.Biophysical journal · 2003Article
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent Ca2+ probes and digital photo-sectioning techniques were used to directly study the dynamics of Ca2+ in isolated mast cell granules of normal (CB/J) and beige (Bg(j)/Bg(j)) mice. The resting intraluminal free Ca2+ concentration ([Ca2+]L) is 25 +/- 4.2 microM (mean +/- SD, n = 68). Exposure to 3 microM inositol 1,4,5-trisphosphate (InsP3) induced periodic oscillations of luminal Ca2+ ([Ca2+]L) of approximately 10 microM amplitude and a period around 8-10 s. The [Ca2+]L oscillations were accompanied by a corresponding oscillatory release of [Ca2+]L to the extraluminal space. Control experiments using ruthenium red (2 microM) and thapsigargin (100 nM) ruled out artifacts derived from the eventual presence of mitochondria or endoplasmic reticulum in the isolated granule preparation. Oscillations of [Ca2+]L and Ca2+ release result from a Ca2+/K+ exchange process whereby bound Ca is displaced from the heparin polyanionic matrix by inflow of K+ into the granular lumen via an apamin-sensitive Ca2+-sensitive K+ channel (ASK(Ca)), whereas Ca2+ release takes place via an InsP3-receptor-Ca2+ (InsP3-R) channel. These results are consistent with previous observations of [Ca2+]L oscillations and release in/from the endoplasmic reticulum and mucin granules, and suggest that a highly conserved common mechanism might be responsible for [Ca2+]L oscillations and quantal periodic Ca2+ release in/from intracellular Ca2+ storage compartments.
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