ArticleGene therapy2001
Direct transfection and activation of human cutaneous dendritic cells.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 71 citations in OpenAlex.
- Nucleic Acid-Based Approaches for Tumor Therapy.Cells · 2020Review
- DNA Vaccines-How Far From Clinical Use?International journal of molecular sciences · 2018Review
- A Topical Mitochondria-Targeted Redox-Cycling Nitroxide Mitigates Oxidative Stress-Induced Skin Damage.The Journal of investigative dermatology · 2017Article
- A Gene Gun-mediated Nonviral RNA trans-splicing Strategy for Col7a1 Repair.Molecular therapy. Nucleic acids · 2016Article
- Dendritic cell-derived interleukin-15 is crucial for therapeutic cancer vaccine potency.Oncoimmunology · 2014Article
- Dissolvable microneedle arrays for intradermal delivery of biologics: fabrication and application.Pharmaceutical research · 2014Article
- CCL19 as an adjuvant for intradermal gene gun immunization in a Her2/neu mouse tumor model: improved vaccine efficacy and a role for B cells as APC.Cancer gene therapy · 2012Article
- Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques.BMC biotechnology · 2011Article
- Development of an ex vivo human skin model for intradermal vaccination: tissue viability and Langerhans cell behaviour.Vaccine · 2009Article
- Physical methods of nucleic acid transfer: general concepts and applications.British journal of pharmacology · 2009Review
- Human skin culture as an ex vivo model for assessing the fibrotic effects of insulin-like growth factor binding proteins.The open rheumatology journal · 2008Article
- DNA vaccines for HIV: challenges and opportunities.Springer seminars in immunopathology · 2006Review
- A differential proteome in tumors suppressed by an adenovirus-based skin patch vaccine encoding human carcinoembryonic antigen.Proteomics · 2005Article
- Plasmid vectors encoding cholera toxin or the heat-labile enterotoxin from Escherichia coli are strong adjuvants for DNA vaccines.Journal of virology · 2002Article
- Development of a Langerhans cell-targeted gene therapy format using a dendritic cell-specific promoter.Gene therapy · 2001Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Gene therapy techniques can be important tools for the induction and control of immune responses. Antigen delivery is a critical challenge in vaccine design, and DNA-based immunization offers an attractive method to deliver encoded transgenic protein antigens. In the present study, we used a gene gun to transfect human skin organ cultures with a particular goal of expressing transgenic antigens in resident cutaneous dendritic cells. Our studies demonstrate that when delivered to human skin, gold particles are observed primarily in the epidermis, even when high helium delivery pressures are used. We demonstrate that Langerhans cells resident in the basal epidermis can be transfected, and that biolistic gene delivery is sufficient to stimulate the activation and migration of skin dendritic cells. RT-PCR analysis of dendritic cells, which have migrated from transfected skin, demonstrates the presence of transgenic mRNA, indicating direct transfection of cutaneous dendritic cells. Importantly, transfected epidermal Langerhans cells can efficiently present a peptide derived from the transgenic melanoma antigen MART-1 to a MART-1-specific CTL. Taken together, our results demonstrate direct transfection, activation, and antigen-specific stimulatory function of in situ transduced human Langerhans cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.