ArticleProceedings of the National Academy of Sciences of the United States of America2001
Controlling small guanine-nucleotide-exchange factor function through cytoplasmic RNA intramers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 93 citations in OpenAlex.
- Chlortetracycline, a Novel Arf Inhibitor That Decreases the Arf6-Dependent Invasive Properties of Breast Cancer Cells.Molecules (Basel, Switzerland) · 2021Article
- Targeting the Small GTPase Superfamily through Their Regulatory Proteins.Angewandte Chemie (International ed. in English) · 2020Review
- Review
- Targeting the polyadenylation factor EhCFIm25 with RNA aptamers controls survival in Entamoeba histolytica.Scientific reports · 2018Article
- Selective aptamer-based control of intraneuronal signaling.Angewandte Chemie (International ed. in English) · 2015Article
- Design and Synthesis of New (SecinH3) Derivatives as Potential Cytohesin Inhibitors.Indian journal of pharmaceutical sciences · 2014Article
- Aptamers: problems, solutions and prospects.Acta naturae · 2013Article
- Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection.PloS one · 2012Article
- Evolution of an inhibitory RNA aptamer against T7 RNA polymerase.FEBS open bio · 2012Article
- Cell-specific aptamers as emerging therapeutics.Journal of nucleic acids · 2011Article
- Ras superfamily GEFs and GAPs: validated and tractable targets for cancer therapy?Nature reviews. Cancer · 2010Review
- Expanding the concept of chemically programmable antibodies to RNA aptamers: chemically programmed biotherapeutics.Angewandte Chemie (International ed. in English) · 2010Article
- Aptamers as therapeutics.Nature reviews. Drug discovery · 2010Review
- FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Induction of distinct TLR2-mediated proinflammatory and proadhesive signaling pathways in response to Porphyromonas gingivalis fimbriae.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Aptamers selected against the unglycosylated EGFRvIII ectodomain and delivered intracellularly reduce membrane-bound EGFRvIII and induce apoptosis.Biological chemistry · 2009Article
- An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro.RNA (New York, N.Y.) · 2008Article
- Structure-based discovery of an inhibitor of Arf activation by Sec7 domains through targeting of protein-protein complexes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of beta-secretase BACE1 in vitro.RNA (New York, N.Y.) · 2006Article
- Co-expression of anti-NFkappaB RNA aptamers and siRNAs leads to maximal suppression of NFkappaB activity in mammalian cells.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ADP-ribosylation factor (ARF) GTPases and their regulatory proteins have been implicated in the control of diverse biological functions. Two main classes of positive regulatory elements for ARF have been discovered so far: the large Sec7/Gea and the small cytohesin/ARNO families, respectively. These proteins harbor guanine-nucleotide-exchange factor (GEF) activity exerted by the common Sec7 domain. The availability of a specific inhibitor, the fungal metabolite brefeldin A, has enabled documentation of the involvement of the large GEFs in vesicle transport. However, because of the lack of such tools, the biological roles of the small GEFs have remained controversial. Here, we have selected a series of RNA aptamers that specifically recognize the Sec7 domain of cytohesin 1. Some aptamers inhibit guanine-nucleotide exchange on ARF1, thereby preventing ARF activation in vitro. Among them, aptamer M69 exhibited unexpected specificity for the small GEFs, because it does not interact with or inhibit the GEF activity of the related Gea2-Sec7 domain, a member of the class of large GEFs. The inhibitory effect demonstrated in vitro clearly is observed as well in vivo, based on the finding that M69 produces similar results as a dominant-negative, GEF-deficient mutant of cytohesin 1: when expressed in the cytoplasm of T-cells, M69 reduces stimulated adhesion to intercellular adhesion molecule-1 and results in a dramatic reorganization of F-actin distribution. These highly specific cellular effects suggest that the ARF-GEF activity of cytohesin 1 plays an important role in cytoskeletal remodeling events of lymphoid cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.