ArticleProceedings of the National Academy of Sciences of the United States of America2001
Mapping of contact sites in complex formation between light-activated rhodopsin and transducin by covalent crosslinking: use of a chemically preactivated reagent.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 99 citations in OpenAlex.
- Mechanistic insights into G-protein coupling with an agonist-bound G-protein-coupled receptor.Nature structural & molecular biology · 2024Article
- Article
- A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gαi1.The Journal of biological chemistry · 2014Article
- GPCR: G protein complexes--the fundamental signaling assembly.Amino acids · 2013Review
- Linking receptor activation to changes in Sw I and II of Gα proteins.Journal of structural biology · 2013Article
- Structural Aspects of GPCR-G Protein Coupling.Toxicological research · 2013Article
- Integrating mass spectrometry of intact protein complexes into structural proteomics.Proteomics · 2012Review
- Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Disease-causing mutation in PKR2 receptor reveals a critical role of positive charges in the second intracellular loop for G-protein coupling and receptor trafficking.The Journal of biological chemistry · 2011Article
- Structural determinants in the second intracellular loop of the human cannabinoid CB1 receptor mediate selective coupling to G(s) and G(i).British journal of pharmacology · 2010Article
- Evolution of a signaling nexus constrained by protein interfaces and conformational States.PLoS computational biology · 2010Article
- An N-terminal polybasic motif of Gαq is required for signaling and influences membrane nanodomain distribution.Molecular pharmacology · 2010Article
- Distinct interactions between the human adrenergic beta(2) receptor and Galpha(s)--an in silico study.Journal of molecular modeling · 2010Article
- Structural basis of G protein-coupled receptor-G protein interactions.Nature chemical biology · 2010Article
- A photocleavable and mass spectrometry identifiable cross-linker for protein interaction studies.Analytical chemistry · 2010Article
- Complexes between photoactivated rhodopsin and transducin: progress and questions.The Biochemical journal · 2010Review
- Finding chimeras: a bioinformatics strategy for identification of cross-linked peptides.Molecular & cellular proteomics : MCP · 2010Article
- Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits.Biochemistry · 2009Article
- Receptor-mediated changes at the myristoylated amino terminus of Galpha(il) proteins.Biochemistry · 2008Article
- Crystal structure of squid rhodopsin with intracellularly extended cytoplasmic region.The Journal of biological chemistry · 2008Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Contact sites in interaction between light-activated rhodopsin and transducin (T) have been investigated by using a chemically preactivated crosslinking reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The 3 propionyl-N-succinimidyl group in the reagent was attached by a disulfide exchange reaction to rhodopsin mutants containing single reactive cysteine groups in the cytoplasmic loops. Complex formation between the derivatized rhodopsin mutants and T was carried out by illumination at lambda > 495 nm. Subsequent increase in pH (from 6 to 7.5 or higher) of the complex resulted in crosslinking of rhodopsin to the T(alpha) subunit. Crosslinking to T(alpha) was demonstrated for the rhodopsin mutants K141C, S240C, and K248C, and the crosslinked sites in T(alpha) were identified for the rhodopsin mutant S240C. The peptides carrying the crosslinking moiety were isolated from the trypsin-digested peptide mixture, and their identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The main site of crosslinking is within the peptide sequence, Leu-19-Arg-28 at the N-terminal region of T(alpha). The total results show that both the N and the C termini of T(alpha) are in close vicinity to the third cytoplasmic loop of rhodopsin in the complex between rhodopsin and T.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.