ArticleProceedings of the National Academy of Sciences of the United States of America2001
Structure and function in rhodopsin: Mass spectrometric identification of the abnormal intradiscal disulfide bond in misfolded retinitis pigmentosa mutants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 66 citations in OpenAlex.
- Expression of red/green-cone opsin mutants K82E, P187S, M273K result in unique pathobiological perturbations to cone structure and function.Frontiers in neuroscience · 2024Article
- Gobind's last graduate student.Biophysical reviews · 2023Article
- Structural aspects of rod opsin and their implication in genetic diseases.Pflugers Archiv : European journal of physiology · 2021Review
- Structure network-based landscape of rhodopsin misfolding by mutations and algorithmic prediction of small chaperone action.Computational and structural biotechnology journal · 2021Article
- A Small Chaperone Improves Folding and Routing of Rhodopsin Mutants Linked to Inherited Blindness.iScience · 2018Article
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- The co-chaperone and reductase ERdj5 facilitates rod opsin biogenesis and quality control.Human molecular genetics · 2014Article
- Inherent instability of the retinitis pigmentosa P23H mutant opsin.The Journal of biological chemistry · 2014Article
- Long range effect of mutations on specific conformational changes in the extracellular loop 2 of angiotensin II type 1 receptor.The Journal of biological chemistry · 2013Article
- Use of proteinase K nonspecific digestion for selective and comprehensive identification of interpeptide cross-links: application to prion proteins.Molecular & cellular proteomics : MCP · 2012Article
- Structure and activation of rhodopsin.Acta pharmacologica Sinica · 2012Review
- Identification of motions in membrane proteins by elastic network models and their experimental validation.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Molecular mechanisms of disease for mutations at Gly-90 in rhodopsin.The Journal of biological chemistry · 2011Article
- Conserved residues in the extracellular loops of short-wavelength cone visual pigments.Biochemistry · 2011Article
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- Conserved rhodopsin intradiscal structural motifs mediate stabilization: effects of zinc.Biochemistry · 2009Article
- A common gene exclusion mechanism used by two chemosensory systems.The European journal of neuroscience · 2009Article
- Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation.Nature structural & molecular biology · 2009Article
- Mechanism of signal propagation upon retinal isomerization: insights from molecular dynamics simulations of rhodopsin restrained by normal modes.Biophysical journal · 2008Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Retinitis pigmentosa (RP) point mutations in both the intradiscal (ID) and transmembrane domains of rhodopsin cause partial or complete misfolding of rhodopsin, resulting in loss of 11-cis-retinal binding. Previous work has shown that misfolding is caused by the formation of a disulfide bond in the ID domain different from the native Cys-110-Cys-187 disulfide bond in native rhodopsin. Here we report on direct identification of the abnormal disulfide bond in misfolded RP mutants in the transmembrane domain by mass spectrometric analysis. This disulfide bond is between Cys-185 and Cys-187, the same as previously identified in misfolded RP mutations in the ID domain. The strategy described here should be generally applicable to identification of disulfide bonds in other integral membrane proteins.
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