ArticleProceedings of the National Academy of Sciences of the United States of America2001
Radial and longitudinal diffusion of myoglobin in single living heart and skeletal muscle cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 76 citations in OpenAlex.
- Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis.BMC molecular and cell biology · 2024Article
- A versatile delivery vehicle for cellular oxygen and fuels or metabolic sensor? A review and perspective on the functions of myoglobin.Physiological reviews · 2024Review
- Scaling of nuclear numbers and their spatial arrangement in skeletal muscle cell size regulation.Molecular biology of the cell · 2023Review
- Capillary-Mitochondrial Oxygen Transport in Muscle: Paradigm Shifts.Function (Oxford, England) · 2023Review
- Oxygen flux from capillary to mitochondria: integration of contemporary discoveries.European journal of applied physiology · 2022Review
- Energy metabolism design of the striated muscle cell.Physiological reviews · 2021Review
- The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin.Scientific reports · 2021Article
- Autonomous microfluidic actuators for periodic sequential flow generation.Science advances · 2019Article
- Insights on the impact of mitochondrial organisation on bioenergetics in high-resolution computational models of cardiac cell architecture.PLoS computational biology · 2018Article
- Differential Interaction of Myoglobin with Select Fatty Acids of Carbon Chain Lengths C8 to C16.Lipids · 2017Article
- A structure-function analysis of the left ventricle.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- Effect of fatty acid interaction on myoglobin oxygen affinity and triglyceride metabolism.Journal of physiology and biochemistry · 2016Article
- Interaction of myoglobin with oleic acid.Chemistry and physics of lipids · 2015Article
- Increased oxidative metabolism and myoglobin expression in zebrafish muscle during chronic hypoxia.Biology open · 2014Article
- Palmitate interaction with physiological states of myoglobin.Biochimica et biophysica acta · 2014Article
- Acute heat stress prior to downhill running may enhance skeletal muscle remodeling.Cell stress & chaperones · 2012Article
- Analysis of molecular movement reveals latticelike obstructions to diffusion in heart muscle cells.Biophysical journal · 2012Article
- Molecules in motion: influences of diffusion on metabolic structure and function in skeletal muscle.The Journal of experimental biology · 2011Review
- Article
- Myoglobin's old and new clothes: from molecular structure to function in living cells.The Journal of experimental biology · 2010Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used a fluorescence recovery after photobleaching (FRAP) technique to measure radial diffusion of myoglobin and other proteins in single skeletal and cardiac muscle cells. We compare the radial diffusivities, D(r) (i.e., diffusion perpendicular to the long fiber axis), with longitudinal ones, D(l) (i.e., parallel to the long fiber axis), both measured by the same technique, for myoglobin (17 kDa), lactalbumin (14 kDa), and ovalbumin (45 kDa). At 22 degrees C, D(l) for myoglobin is 1.2 x 10(-7) cm(2)/s in soleus fibers and 1.1 x 10(-7) cm(2)/s in cardiomyocytes. D(l) for lactalbumin is similar in both cell types. D(r) for myoglobin is 1.2 x 10(-7) cm(2)/s in soleus fibers and 1.1 x 10(-7) cm(2)/s in cardiomyocytes and, again, similar for lactalbumin. D(l) and D(r) for ovalbumin are 0.5 x 10(-7) cm(2)/s. In the case of myoglobin, both D(l) and D(r) at 37 degrees C are about 80% higher than at 22 degrees C. We conclude that intracellular diffusivity of myoglobin and other proteins (i) is very low in striated muscle cells, approximately 1/10 of the value in dilute protein solution, (ii) is not markedly different in longitudinal and radial direction, and (iii) is identical in heart and skeletal muscle. A Krogh cylinder model calculation holding for steady-state tissue oxygenation predicts that, based on these myoglobin diffusivities, myoglobin-facilitated oxygen diffusion contributes 4% to the overall intracellular oxygen transport of maximally exercising skeletal muscle and less than 2% to that of heart under conditions of high work load.
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