ArticleOncogene2001
Phosphorylation of serines 15 and 37 is necessary for efficient accumulation of p53 following irradiation with UV.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Protein Phosphatase 2ACα Regulates ATR-Mediated Endogenous DNA Damage Response Against Microcephaly.Molecular neurobiology · 2025Article
- IL-24 Promotes Apoptosis through cAMP-Dependent PKA Pathways in Human Breast Cancer Cells.International journal of molecular sciences · 2018Article
- Role of p38 and JNK MAPK signaling pathways and tumor suppressor p53 on induction of apoptosis in response to Ad-eIF5A1 in A549 lung cancer cells.Molecular cancer · 2013Article
- Ubiquitin proteasomal pathway mediated degradation of p53 in melanoma.Archives of biochemistry and biophysics · 2011Article
- Curcuminoids activate p38 MAP kinases and promote UVB-dependent signalling in keratinocytes.Experimental dermatology · 2010Article
- Regulation of p53 expression, phosphorylation and subcellular localization by a G-protein-coupled receptor.Oncogene · 2009Article
- Logical network of genotoxic stress-induced NF-κB signal transduction predicts putative target structures for therapeutic intervention strategies.Advances and applications in bioinformatics and chemistry : AABC · 2009Article
- Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.Journal of virology · 2009Article
- 15-deoxy-Delta(12,14)-prostaglandin J2 induces vascular endothelial cell apoptosis through the sequential activation of MAPKS and p53.The Journal of biological chemistry · 2008Article
- The role of serine/threonine protein phosphatase type 5 (PP5) in the regulation of stress-induced signaling networks and cancer.Cancer metastasis reviews · 2008Review
- Reactivation of p53 in cells expressing hepatitis B virus X-protein involves p53 phosphorylation and a reduction of Hdm2.Cancer science · 2008Article
- Elevated levels of Ser/Thr protein phosphatase 5 (PP5) in human breast cancer.Biochimica et biophysica acta · 2008Article
- Loss of one p53 allele results in four-fold reduction of p53 mRNA and protein: a basis for p53 haplo-insufficiency.Oncogene · 2006Article
- Subcellular targeting of p33ING1b by phosphorylation-dependent 14-3-3 binding regulates p21WAF1 expression.Molecular and cellular biology · 2006Article
- Defect in serine 46 phosphorylation of p53 contributes to acquisition of p53 resistance in oral squamous cell carcinoma cells.Oncogene · 2006Article
- Nuclear accumulation of p53 following inhibition of transcription is not due to diminished levels of MDM2.Oncogene · 2004Article
- Limited role of N-terminal phosphoserine residues in the activation of transcription by p53.Oncogene · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Changes in the phosphorylation state of p53 are important in increasing its half-life and potency as a transcription factor. To investigate their roles, serine residues 15 and 37 were mutated to alanines and the mutated proteins were expressed stably at low basal levels in Li-Fraumeni-derived p53-null human fibroblasts. The accumulation of p53 after DNA damage was analysed quantitatively in multiple clones. Mutation of serine 15, serine 37 or both impaired the accumulation of the protein after exposing the cells to ultraviolet radiation (50-100% increase for the mutant proteins, 500% increase for wild-type p53) but not after treatment with adriamycin. The diminished accumulation of mutant p53 protein is due to a reduction of basal HDM association. Analysis of p53-dependent transcription revealed that phosphorylation of serine 15 is required to maintain basal levels of p21 mRNA. These results provide new evidence for an important function of serine 37 phosphorylation, clearly distinguish the pathways of p53 activation in response to ultraviolet radiation or DNA damage inflicted by adriamycin, and reveal that serine 15 is crucial to support the p53-mediated basal expression of p21.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.