ArticleOncogene2001
An hsRPB4/7-dependent yeast assay for trans-activation by the EWS oncogene.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Never a dull enzyme, RNA polymerase II.Transcription · 2023Review
- Targeted Therapy for EWS-FLI1 in Ewing Sarcoma.Cancers · 2023Review
- Review
- Structure-function based molecular relationships in Ewing's sarcoma.BioMed research international · 2015Review
- Ewing sarcoma protein: a key player in human cancer.International journal of cell biology · 2013Review
- The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.Protein & cell · 2012Article
- Factors Affecting EWS-FLI1 Activity in Ewing's Sarcoma.Sarcoma · 2011Article
- A transcription assay for EWS oncoproteins in Xenopus oocytes.Protein & cell · 2010Article
- The TET family of proteins: functions and roles in disease.Journal of molecular cell biology · 2009Review
- In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II hsRPB7.Molecular biology reports · 2009Article
- Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.The Biochemical journal · 2008Article
- Multiple aromatic side chains within a disordered structure are critical for transcription and transforming activity of EWS family oncoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.Nucleic acids research · 2005Article
- RGG-boxes of the EWS oncoprotein repress a range of transcriptional activation domains.Nucleic acids research · 2005Article
- Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III.Molecular and cellular biology · 2003Article
- A repetitive element containing a critical tyrosine residue is required for transcriptional activation by the EWS/ATF1 oncogene.Oncogene · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomal fusions of the N-terminal region of the Ewings Sarcoma Oncogene (EWS-Activation-Domain, EAD) to the DNA-binding domains of a variety of cellular transcription factors, produce oncogenic proteins (EWS-fusion proteins (EFPs)) that cause a variety of malignancies. The EAD can act as a potent transcriptional activation domain and is required for the oncogenic activity of EFPs. Previous studies demonstrating a physical interaction between the EAD and the human RNA Polymerase II subunit hsRPB7 suggest a crucial role for RPB7 and its partner, RPB4, in EAD function. Homologues of hsRPB4/7 exist in S. cerevisiae, and here we describe an RPB4/7-dependent yeast assay for EAD-mediated trans-activation. Conditional yeast strains lacking RPB4 are defective for trans-activation by a Gal4/EAD fusion protein at the permissive temperature. Introduction of hsRPB4 alone is unable to rescue trans-activation, while a combination of hsRPB4 and hsRPB7 significantly rescues activity. These findings provide the first functional evidence for a direct role of the RPB4/7 complex in EAD-mediated trans-activation. In addition, the yeast assay provides a tractable system for further molecular analysis of EAD and RPB4/7 action.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.