ArticleGene therapy2001
Influence of multiplicity of infection and protein stability on retroviral vector-mediated gene expression in hematopoietic cells.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 74 citations in OpenAlex.
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- Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.Development (Cambridge, England) · 2016Article
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- MicroRNA-224 Induces G1/S Checkpoint Release in Liver Cancer.Journal of clinical medicine · 2015Article
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- A unique carboxyl-terminal insert domain in the hematopoietic-specific, GTPase-deficient Rho GTPase RhoH regulates post-translational processing.The Journal of biological chemistry · 2013Article
- Coordinated effects of microRNA-494 induce G₂/M arrest in human cholangiocarcinoma.Cell cycle (Georgetown, Tex.) · 2012Article
- MicroRNA down-regulated in human cholangiocarcinoma control cell cycle through multiple targets involved in the G1/S checkpoint.Hepatology (Baltimore, Md.) · 2011Article
- Improved Tet-responsive promoters with minimized background expression.BMC biotechnology · 2010Article
- RhoH regulates subcellular localization of ZAP-70 and Lck in T cell receptor signaling.PloS one · 2010Article
- Self-inactivating alpharetroviral vectors with a split-packaging design.Journal of virology · 2010Article
- Enhanced homing and engraftment of fresh but not ex vivo cultured murine marrow cells in submyeloablated hosts following CD26 inhibition by Diprotin A.Experimental hematology · 2009Article
- A novel competitive repopulation strategy to quantitate engraftment of ex vivo manipulated murine marrow cells in submyeloablated hosts.Experimental hematology · 2008Article
- In vivo selection of hematopoietic stem cells transduced at a low multiplicity-of-infection with a foamy viral MGMT(P140K) vector.Experimental hematology · 2008Article
- Vector design for expression of O6-methylguanine-DNA methyltransferase in hematopoietic cells.DNA repair · 2007Review
- T-bet is a critical determinant in the instability of the IL-17-secreting T-helper phenotype.Blood · 2006Article
- Article
- Optimization of equine infectious anemia derived vectors for hematopoietic cell lineage gene transfer.Gene therapy · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using retroviral vectors encoding enhanced green fluorescent protein (EGFP), we addressed to what extent expression of retroviral transgenes in hematopoietic cells depends on the multiplicity of infection (MOI) and on the half-life of the encoded protein. We show that an elevation of the MOI not only elevates the frequency of transduced cells, but also increases transgene expression levels and reduces interanimal variability in vivo (hematopoietic cells of C57BL/6J mice analyzed 13 weeks after transplantation). This suggests that the MOI has to be carefully controlled and should be adapted as desired for clinical studies when evaluating vector performance in preclinical models. The impact of protein stability is demonstrated by comparing vectors expressing EGFP or a destabilized variant with a C-terminal PEST-sequence, d2EGFP. The loss of expression with d2EGFP was more pronounced in terminally differentiated cells of the peripheral blood (>30 fold) than in progenitor cells (five- to 10-fold), indicating a stronger transcription of the retroviral promoter in progenitor cells and a predominant role of protein inheritance over de novo synthesis of transgenic protein in mature blood cells. This analysis reveals an important and differentiation-dependent contribution of protein half-life to the expression of retroviral vectors in hematopoietic cells, establishes d2EGFP as a more accurate reporter for determination of vector transcription, and also suggests that preclinical data obtained under conditions of high transduction rates or with vectors expressing stable reporter proteins require careful interpretation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.