ArticleMolecular and cellular biology2001
Localization of the Rsp5p ubiquitin-protein ligase at multiple sites within the endocytic pathway.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 90 citations in OpenAlex.
- The role of HECT-type E3 ubiquitin ligases in inflammation.Frontiers in immunology · 2026Review
- Poly-ubiquitylated transmembrane proteins outcompete other cargo for limited space inside clathrin-coated vesicles.Cell reports · 2025Article
- Article
- The ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis.The Journal of biological chemistry · 2014Article
- Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination.Current biology : CB · 2014Article
- Article
- Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals.European journal of cell biology · 2011Article
- Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch.Journal of virology · 2010Article
- Eps15: a multifunctional adaptor protein regulating intracellular trafficking.Cell communication and signaling : CCS · 2009Article
- Gly-46 and His-50 of yeast maltose transporter Mal21p are essential for its resistance against glucose-induced degradation.The Journal of biological chemistry · 2009Article
- Article
- Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome.Molecular biology of the cell · 2008Article
- Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies.Molecular biology of the cell · 2008Article
- Article
- Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p.Molecular biology of the cell · 2007Article
- Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway.Molecular biology of the cell · 2007Article
- Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Drosophila melanogaster.Molecular and cellular biology · 2007Article
- Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies.Molecular biology of the cell · 2007Article
- Novel interaction between Apc5p and Rsp5p in an intracellular signaling pathway in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- Late assembly motifs of human T-cell leukemia virus type 1 and their relative roles in particle release.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The Saccharomyces cerevisiae RSP5 gene encodes an essential HECT E3 ubiquitin-protein ligase. Rsp5p contains an N-terminal C2 domain, three WW domains in the central portion of the molecule, and a C-terminal catalytic HECT domain. A diverse group of substrates of Rsp5p and vertebrate C2 WW-domain-containing HECT E3s have been identified, including both nuclear and membrane-associated proteins. We determined the intracellular localization of Rsp5p and the determinants necessary for localization, in order to better understand how Rsp5p activities are coordinated. Using both green fluorescent protein fusions to Rsp5p and immunogold electron microscopy, we found that Rsp5p was distributed in a punctate pattern at the plasma membrane, corresponding to membrane invaginations that are likely sites of endosome formation, as well as at perivacuolar sites. The latter appeared to correspond to endocytic intermediates, as these structures were not seen in a sla2/end4-1 mutant, and double-immunogold labeling demonstrated colocalization of Rsp5p with the endosomal markers Pep12p and Vps32p. The C2 domain was an important determinant of localization; however, mutations that disrupted HECT domain function also caused mislocalization of Rsp5p, indicating that enzymatic activity is linked to localization. Deletion of the C2 domain partially stabilized Fur4p, a protein previously shown to undergo Rsp5p- and ubiquitin-mediated endocytosis; however, Fur4p was still ubiquitinated at the plasma membrane when the C2 domain was deleted from the protein. Together, these results indicate that Rsp5p is located at multiple sites within the endocytic pathway and suggest that Rsp5p may function at multiple steps in the ubiquitin-mediated endocytosis pathway.
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