ArticleMolecular and cellular biology2001
A novel form of transcriptional silencing by Sum1-1 requires Hst1 and the origin recognition complex.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 85 citations in OpenAlex.
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- The Agrobacterium VirE2 effector interacts with multiple members of the Arabidopsis VIP1 protein family.Molecular plant pathology · 2018Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
- Adaptor proteins GIR1 and GIR2. I. Interaction with the repressor GLABRA2 and regulation of root hair development.Biochemical and biophysical research communications · 2017Article
- Determinants of Sir2-Mediated, Silent Chromatin Cohesion.Molecular and cellular biology · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- The transcriptional repressor Sum1p counteracts Sir2p in regulation of the actin cytoskeleton, mitochondrial quality control and replicative lifespan inMicrobial cell (Graz, Austria) · 2016Article
- Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein.Scientific reports · 2015Article
- Less is more: Nutrient limitation induces cross-talk of nutrient sensing pathways with NADFrontiers in biology · 2015Article
- Sirtuins in epigenetic regulation.Chemical reviews · 2015Review
- Interaction of Arabidopsis Trihelix-Domain Transcription Factors VFP3 and VFP5 with Agrobacterium Virulence Protein VirF.PloS one · 2015Article
- Regulation of NAD+ metabolism, signaling and compartmentalization in the yeast Saccharomyces cerevisiae.DNA repair · 2014Review
- Interaction between the transcriptional corepressor Sin3B and voltage-gated sodium channels modulates functional channel expression.Scientific reports · 2013Article
- Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1.Current biology : CB · 2013Article
- Biochemical characterization of Hpa2 and Hpa3, two small closely related acetyltransferases from Saccharomyces cerevisiae.The Journal of biological chemistry · 2013Article
- Genome-wide analysis of functional sirtuin chromatin targets in yeast.Genome biology · 2013Article
- A plasmodesmal glycosyltransferase-like protein.PloS one · 2013Article
- The Sum1/Ndt80 transcriptional switch and commitment to meiosis in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2012Review
- Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast.Molecular and cellular biology · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
In the yeast Saccharomyces cerevisiae, a and alpha mating-type information is stored in transcriptionally silenced cassettes called HML and HMR. Silencing of these loci, maintained by the formation of a specialized type of heterochromatin, requires trans-acting proteins and cis-acting elements. Proteins required for silencing include the Sir2 NAD(+)-dependent deacetylase, Sir3, and Sir4. Factors that bind to the cis elements at HMR and HML and that are important for silencing include the origin recognition complex (ORC). Mutations of any of these Sir proteins or combinations of cis elements result in loss of silencing. SUM1-1 was previously identified as a dominant mutation that restores silencing to HMR in the absence of either the Sir proteins or some of the cis elements. We have investigated the novel mechanism whereby Sum1-1 causes Sir-independent silencing at HMR and present the following findings: Sum1-1 requires the Sir2 homolog, Hst1, for silencing and most probably requires the NAD(+)-dependent deacetylase activity of this protein. Sum1-1 interacts strongly with ORC, and this strong interaction is dependent on HMR DNA. Furthermore, ORC is required for Sum1-1-mediated silencing at HMR. These observations lead to a model for Sum1-1 silencing of HMR in which Sum1-1 is recruited to HMR by binding to ORC. Sum1-1, in turn, recruits Hst1. Hst1 then deacetylates histones or other chromatin-associated proteins to cause chromatin condensation and transcriptional silencing.
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