ArticleMolecular and cellular biology2001
Protein binding protects sites on stable episomes and in the chromosome from de novo methylation.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- How do lifestyle and environmental factors influence the sperm epigenome? Effects on sperm fertilising ability, embryo development, and offspring health.Clinical epigenetics · 2025Review
- Article
- SEMplMe: a tool for integrating DNA methylation effects in transcription factor binding affinity predictions.BMC bioinformatics · 2022Article
- Methylation in the Promoter Region of the Dopamine TransporterInternational journal of environmental research and public health · 2022Article
- Pioneer transcription factors are associated with the modulation of DNA methylation patterns across cancers.Epigenetics & chromatin · 2022Article
- Review
- Epigenetic Reprogramming of Tumor-Associated Fibroblasts in Lung Cancer: Therapeutic Opportunities.Cancers · 2021Review
- Molecular and computational approaches to map regulatory elements in 3D chromatin structure.Epigenetics & chromatin · 2021Review
- Validated inference of smoking habits from blood with a finite DNA methylation marker set.European journal of epidemiology · 2019Article
- Gene activation precedes DNA demethylation in response to infection in human dendritic cells.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Epigenetic Regulations in Neuropsychiatric Disorders.Frontiers in genetics · 2019Review
- Methylation-Demethylation Dynamics: Implications of Changes in Acute Kidney Injury.Analytical cellular pathology (Amsterdam) · 2018Review
- DNA methylation and DNA methyltransferases.Epigenetics & chromatin · 2017Review
- Transcription factors as readers and effectors of DNA methylation.Nature reviews. Genetics · 2016Review
- Notes on the role of dynamic DNA methylation in mammalian development.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- Human lymphoid translocation fragile zones are hypomethylated and have accessible chromatin.Molecular and cellular biology · 2015Article
- Prenatal exposure to maternal cigarette smoking and DNA methylation: epigenome-wide association in a discovery sample of adolescents and replication in an independent cohort at birth through 17 years of age.Environmental health perspectives · 2015Article
- Function and information content of DNA methylation.Nature · 2015Review
- The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice.Molecular and cellular biology · 2013Article
- Cigarette smoking and DNA methylation.Frontiers in genetics · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have utilized the Escherichia coli lac repressor-operator system to test whether protein binding can interfere with de novo DNA methylation in mammalian cells. We find that a DNA binding protein can protect sites on the episome as well as in the genome from the de novo methylation activity of Dnmt3a. Transcriptional machinery moving through the binding sites does not affect the de novo methylation of these sites, and it does not affect the binding protein protection of these sites from de novo methylation. This study and previous studies provide a possible mechanism for the observation that an Sp1 site can serve as a cis-acting signal for demethylation and for preventing de novo methylation of the CpG island upstream of the mouse adenine phosphoribosyltransferase (Aprt) gene. These findings also support the hypothesis that protein binding may play a crucial role in changes of CpG methylation pattern in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.