ArticleThe Journal of physiology2001
Sustained stimulation of exocytosis triggers continuous membrane retrieval in rat pituitary somatotrophs.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Resistance exercise stress: theoretical mechanisms for growth hormone processing and release from the anterior pituitary somatotroph.European journal of applied physiology · 2023Review
- Alcohol metabolite acetic acid activates BK channels in a pH-dependent manner and decreases calcium oscillations and exocytosis of secretory granules in rat pituitary GH3 cells.Pflugers Archiv : European journal of physiology · 2021Article
- Insights into the Nanobiology of Growth Hormone Secretion.Discoveries (Craiova, Romania) · 2014Article
- Extrasynaptic exocytosis and its mechanisms: a source of molecules mediating volume transmission in the nervous system.Frontiers in physiology · 2012Article
- Colocalization of FM1-43, Bassoon, and GnRH-1: GnRH-1 release from cell bodies and their neuroprocesses.Endocrinology · 2011Article
- Evidence for AMPK-dependent regulation of exocytosis of lipoproteins in a model liver cell line.Experimental cell research · 2008Article
- Dual control of cardiac Na+ Ca2+ exchange by PIP(2): electrophysiological analysis of direct and indirect mechanisms.The Journal of physiology · 2007Article
- Inhibition of cellular responses to insulin in a rat liver cell line. A role for PKC in insulin resistance.The Journal of physiology · 2005Article
- Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro.The Journal of membrane biology · 2005Article
- Retention of peptide hormones during partial secretion in pituitary somatotrophs and corticotrophs.Pflugers Archiv : European journal of physiology · 2005Article
- Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones.The Journal of physiology · 2003Article
- Calcium-dependent exocytosis of atrial natriuretic peptide from astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Exocytosis in bovine chromaffin cells: studies with patch-clamp capacitance and FM1-43 fluorescence.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the relationship between exocytosis and endocytosis in rat pituitary somatotrophs using patch-clamp capacitance, FM1-43 fluorescence imaging and amperometry. Stimulation of exocytosis through voltage-dependent Ca2+ channels by depolarizations (1-5 s) increased the capacitance by 4.3 +/- 0.9 % and the fluorescence by 6.6 +/- 1.1 % (10 cells). The correlation between the capacitance and fluorescence changes indicated that the cell membrane and granule membrane added via exocytosis were stained with the membrane-bound fluorescent dye FM1-43 in a quantitatively similar manner. Intracellular dialysis (0.5-4.5 min) with elevated Ca2+ (1.5-100 microM) evoked continuous exocytosis that was detected with a carbon fibre electrode from dopamine-loaded cells (10 cells) or as an increase in FM1-43 fluorescence (56 +/- 10 %; 21 cells). Interestingly during Ca2+ dialysis the capacitance did not significantly change (2 +/- 1 %; 31 cells), indicating that endocytosis efficiently retrieved increased cell membrane. Sustained endocytosis was not blocked when the intracellular GTP (300 microM) was replaced with GTP[gamma]S. Replacing intracellular Ca2+ (100 microM) with Ba2+ (300 microM) or Sr2+ (200 microM), or reducing the pH of the intracellular solution from 7.2 to 6.2 did not block sustained endocytosis. Our results suggest that pituitary somatotrophs have the ability to undergo continuous exocytosis and membrane retrieval that persist in whole-cell recordings.
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