ArticleThe Journal of physiology2001
Involvement of Rho-kinase and tyrosine kinase in hypotonic stress-induced ATP release in bovine aortic endothelial cells.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 100 citations in OpenAlex.
- Osmotic pressure-induced calcium response states.FEBS open bio · 2025Article
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- Extracellular purines in lung endothelial permeability and pulmonary diseases.Frontiers in physiology · 2024Review
- Physiological Functions of the Volume-Regulated Anion Channel VRAC/LRRC8 and the Proton-Activated Chloride Channel ASOR/TMEM206.Handbook of experimental pharmacology · 2024Article
- Pannexin 1, a large-pore membrane channel, contributes to hypotonicity-induced ATP release in Schwann cells.Neural regeneration research · 2021Article
- Review
- Review
- Detection of Osmotic Shock-Induced Extracellular Nucleotide Release with a Genetically Encoded Fluorescent Sensor of ADP and ATP.Sensors (Basel, Switzerland) · 2019Article
- Rapid disruption of intestinal epithelial tight junction and barrier dysfunction by ionizing radiation in mouse colon in vivo: protection by N-acetyl-l-cysteine.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Hypoxia is an effective stimulus for vesicular release of ATP from human umbilical vein endothelial cells.Placenta · 2015Article
- Hypotonic stress induces RANKL via transient receptor potential melastatin 3 (TRPM3) and vaniloid 4 (TRPV4) in human PDL cells.Journal of dental research · 2015Article
- Nitric oxide enhances keratinocyte cell migration by regulating Rho GTPase via cGMP-PKG signalling.PloS one · 2015Article
- Possible contribution of pannexin-1 to ATP release in human upper airway epithelia.Physiological reports · 2014Article
- Fibroblast cytoskeletal remodeling induced by tissue stretch involves ATP signaling.Journal of cellular physiology · 2013Article
- Cellular control of connective tissue matrix tension.Journal of cellular biochemistry · 2013Review
- Involvement of connexin43 hemichannel in ATP release after γ-irradiation.Journal of radiation research · 2012Article
- Rho signaling regulates pannexin 1-mediated ATP release from airway epithelia.The Journal of biological chemistry · 2011Article
- Nucleotide receptors as targets in the pharmacological enhancement of dermal wound healing.Purinergic signalling · 2011Article
- Initiation of purinergic signaling by exocytosis of ATP-containing vesicles in liver epithelium.The Journal of biological chemistry · 2010Article
- Extracellular osmolarity modulates G protein-coupled receptor-dependent ATP release from 1321N1 astrocytoma cells.American journal of physiology. Cell physiology · 2010Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypotonic stress induces ATP release followed by Ca2+ oscillations in bovine aortic endothelial cells (BAECs). We have investigated the cellular mechanism of the hypotonic stress-induced ATP release. Hypotonic stress induced tyrosine phosphorylation of at least two proteins, of 110 and 150 kDa. Inhibition of tyrosine kinase by the tyrosine kinase inhibitors herbimycin A and tyrphostin 46 prevented ATP release and ATP-mediated Ca2+ oscillations induced by hypotonic stress. ATP release was also inhibited by the pretreatment of the cells with botulinum toxin C3, and augmented by lysophosphatidic acid. Furthermore, pre-treating the cells with Y-27632, a selective inhibitor of Rho-kinase, also suppressed the hypotonic stress-induced ATP release and Ca2+ oscillations, indicating that Rho-mediated activation of Rho-kinase may be involved in the hypotonic ATP release. Hypotonic stress also induced a transient rearrangement of the actin cytoskeleton, which was suppressed by the tyrosine kinase inhibitors Y-27632 and cytochalasin B. However, pretreatment of the cell with cytochalasin B inhibited neither the hypotonic stress-induced ATP release nor the Ca2+ oscillations. These results indicate that tyrosine kinase and the Rho-Rho-kinase pathways are involved in hypotonic stress-induced ATP release and actin rearrangement, but actin polymerization is not required for ATP release in BAECs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.