ArticleThe Journal of physiology2001
Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Monomethyl auristatin E and paclitaxel use different mechanisms to alter intracellular calcium signaling.Biochemical pharmacology · 2025Article
- The Janus-faced functions of Apolipoproteins L in membrane dynamics.Cellular and molecular life sciences : CMLS · 2024Article
- A novel α-conotoxin [D1G, ΔQ14] LvIC decreased mouse locomotor activity.Frontiers in pharmacology · 2024Article
- Dopamine D2 autoreceptor interactome: Targeting the receptor complex as a strategy for treatment of substance use disorder.Pharmacology & therapeutics · 2020Review
- Current Understanding of the Role of Neuronal Calcium Sensor 1 in Neurological Disorders.Molecular neurobiology · 2019Review
- Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Multiple roles for frequenin/NCS-1 in synaptic function and development.Molecular neurobiology · 2012Review
- Neuronal calcium sensor-1 regulation of calcium channels, secretion, and neuronal outgrowth.Cellular and molecular neurobiology · 2010Review
- Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.PLoS biology · 2010Article
- Structural and functional deficits in a neuronal calcium sensor-1 mutant identified in a case of autistic spectrum disorder.PloS one · 2010Article
- ROS-GC subfamily membrane guanylate cyclase-linked transduction systems: taste, pineal gland and hippocampus.Molecular and cellular biochemistry · 2010Review
- Molecular characterization of the Aspergillus fumigatus NCS-1 homologue, NcsA.Molecular genetics and genomics : MGG · 2008Article
- Novel frequenin-modulated Ca2+-signaling membrane guanylate cyclase (ROS-GC) transduction pathway in bovine hippocampus.Molecular and cellular biochemistry · 2006Article
- Novel role of neuronal Ca2+ sensor-1 as a survival factor up-regulated in injured neurons.The Journal of cell biology · 2006Article
- Neuronal calcium sensor-1 potentiates glucose-dependent exocytosis in pancreatic beta cells through activation of phosphatidylinositol 4-kinase beta.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Functional properties of motoneurons derived from mouse embryonic stem cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Expression and possible role of neuronal calcium sensor-1 in the cerebellum.Cerebellum (London, England) · 2004Review
- Identification and subcellular localization of neuronal calcium sensor-1 (NCS-1) in human neutrophils and HL-60 cells.Inflammation · 2003Article
- Up-regulation of neuronal calcium sensor-1 (NCS-1) in the prefrontal cortex of schizophrenic and bipolar patients.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Activity-dependent regulation of the subcellular localization of neuronal calcium sensor-1 in the avian cochlear nucleus.Neuroscience · 2003Article
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Authors and funding
9 authors.
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Abstract
The role of rat neuronal calcium sensor-1 (NCS-1), a Ca2+-binding protein, in synapse formation and transmitter release was examined in mouse neuroblastoma x rat glioma hybrid NG108-15 cells in culture. Wild-type NG108-15 cells expressed rodent NCS-1. Endogenous NCS-1 was partially co-localized with the synaptic protein SNAP-25 at the plasma membrane in both cell bodies and processes, but not with the Golgi marker [beta]-COP, an individual coat subunit of the coatomer complex present on Golgi-derived vesicles. In NG108-15 cells co-cultured with rat myotubes, partial co-localization of SNAP-25 and NCS-1 was observed at the plasma membrane of neurites and growth cones, some of which had synaptic contacts to muscle cells. Transient co-transfection of the rat NCS-1 cDNA and green fluorescent protein (GFP) resulted in NCS-1 overexpression in about 30 % of the cells as determined by fluorescence microscopy. The rate of functional synapse formation with co-cultured rat myotubes increased 2-fold as determined by the presence of miniature endplate potentials (MEPPs) in NCS-1-overexpressing NG108-15 cells compared to non- and mock-transfected cells. The number of neurites per cell, branches per neurite and length of neurites was slightly less in cells that were either transiently transfected (GFP-NCS-1-fluorescence positive) or stably transformed with NCS-1 compared to GFP-NCS-1-negative, non-transfected or mock-transfected NG108-15 cells. The number of action potentials that elicited endplate potentials increased in NG108-15 cells stably transformed with rat NCS-1. The mean number of quanta per impulse (m) increased 5-fold. These results show that NCS-1 functions to facilitate synapse formation, probably because of the increased quantal content of evoked acetylcholine release.
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