ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Excitatory nicotinic and desensitizing muscarinic (M2) effects on C-nociceptors in isolated rat skin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 113 citations in OpenAlex.
- Smoking Abstinence Elicits Hyperalgesia: Neural Mechanism, Clinical Implications, and Pharmacological Managements.CNS neuroscience & therapeutics · 2026Review
- Peripheral Parasympathetic Networks in Pain: A Systems-Level Review of Mechanisms and Modulations.Cellular and molecular neurobiology · 2025Review
- Nicotine induces abnormal motor coupling through sensitization of a mechanosensory circuit in Caenorhabditis elegans.PLoS biology · 2025Article
- Pain Management with Natural Products: Neurophysiological Insights.International journal of molecular sciences · 2025Review
- Sensory Effects of Nicotine and Tobacco.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2022Review
- Nicotine Evoked Currents in Human Primary Sensory Neurons.The journal of pain · 2019Article
- Decanoic acid attenuates the excitability of nociceptive trigeminal primary and secondary neurons associated with hypoalgesia.Journal of pain research · 2018Article
- Commentary: Cholinergic Nociceptive Mechanisms in Rat Meninges and Trigeminal Ganglia: Potential Implications for Migraine Pain.Frontiers in neurology · 2017Article
- Cholinergic Nociceptive Mechanisms in Rat Meninges and Trigeminal Ganglia: Potential Implications for Migraine Pain.Frontiers in neurology · 2017Article
- Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.Neuroscience · 2015Article
- Artemin growth factor increases nicotinic cholinergic receptor subunit expression and activity in nociceptive sensory neurons.Molecular pain · 2014Article
- Caenorhabditis elegans nicotinic acetylcholine receptors are required for nociception.Molecular and cellular neurosciences · 2014Article
- Libidibia ferrea mature seeds promote antinociceptive effect by peripheral and central pathway: possible involvement of opioid and cholinergic receptors.BioMed research international · 2014Article
- New pharmacological approaches to the cholinergic system: an overview on muscarinic receptor ligands and cholinesterase inhibitors.Recent patents on CNS drug discovery · 2013Review
- Effects of nicotine on spinal cord injury pain: a randomized, double-blind, placebo controlled crossover trial.Topics in spinal cord injury rehabilitation · 2012Article
- The sympathetic nervous system and pain.Neuromolecular medicine · 2008Review
- [Cholinergic receptors of sensory neurons of the urinary bladder].Der Urologe. Ausg. A · 2007Article
- Ca2+ permeability of nicotinic acetylcholine receptors from rat dorsal root ganglion neurones.The Journal of physiology · 2005Article
- Nicotine increases initial blood flow responses to local heating of human non-glabrous skin.The Journal of physiology · 2004Article
- Nicotinic receptor alpha 7-subunits are coupled to the stimulation of nitric oxide synthase in rat dorsal root ganglion neurons.Histochemistry and cell biology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actions of different cholinergic agonists and antagonists were investigated on nociceptive afferents using the rat skin-saphenous nerve preparation, in vitro. Nicotine was able to weakly excite C-nociceptors and to induce a mild sensitization to heat stimulation (in 77% of tested fibers) in a dose-dependent manner (10(-)6 to 10(-)5 m), but it caused no alteration in mechanical responsiveness tested with von Frey hairs. Muscarine did not induce a significant nociceptor excitation, but almost all fibers exhibited a marked desensitization to mechanical and heat stimuli in a dose-dependent manner (from 10(-)6 to 10(-)4 m). The muscarinic effects could be prevented by the general muscarinic antagonist scopolamine (10(-)5 m), by the M3 antagonist 1,1-dimethyl-4-diphenylacetoxypiperidium oxide (10(-)6 m) co-applied with the M2 antagonist gallamine (10(-)5 m), and by gallamine alone. As positive control we used the relatively M2-selective agonist arecaidine (10(-)6 to 10(-)5 m), obtaining a similar desensitizing effect as with muscarine. Finally, we performed an immunocytochemical study that demonstrated the presence of M2 but not M3 receptors in thin epidermal nerve fibers of the rat hairy skin. Altogether, these data demonstrate opposite effects of nicotinic and muscarinic receptor stimulation on cutaneous nociceptors. M2 receptor-mediated depression of nociceptive responsiveness may convey a therapeutic, i.e., analgesic or antinociceptive, potential.
Indexed as
Identifiers
11312314PMC6762575W2109955163What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.