ArticleProceedings of the National Academy of Sciences of the United States of America2001
Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 214 citations in OpenAlex.
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- Vaginal Isolates of Candida glabrata Are Uniquely Susceptible to Ionophoric Killer Toxins Produced by Saccharomyces cerevisiae.Antimicrobial agents and chemotherapy · 2021Article
- Bisphosphonate inhibitors of squalene synthase protect cells against cholesterol-dependent cytolysins.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Function of Platelet Glycosphingolipid Microdomains/Lipid Rafts.International journal of molecular sciences · 2020Review
- Mammalian membrane trafficking as seen through the lens of bacterial toxins.Cellular microbiology · 2020Review
- Brain sterol flux mediated by cytochrome P450 46A1 affects membrane properties and membrane-dependent processes.Brain communications · 2020Article
- Membrane Binding, Cellular Cholesterol Content and Resealing Capacity Contribute to Epithelial Cell Damage Induced by Suilysin ofPathogens (Basel, Switzerland) · 2019Article
- Cholesterol-Dependent Cytolysins Produced by Vaginal Bacteria: Certainties and Controversies.Frontiers in cellular and infection microbiology · 2019Review
- Pneumolysin-damaged cells benefit from non-homogeneous toxin binding to cholesterol-rich membrane domains.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Interaction of Cholesterol with Perfringolysin O: What Have We Learned from Functional Analysis?Toxins · 2017Review
- Roles of the Mevalonate Pathway and Cholesterol Trafficking in Pulmonary Host Defense.Current molecular pharmacology · 2017Article
- Distinct surveillance pathway for immunopathology during acute infection via autophagy and SR-BI.Scientific reports · 2016Article
- Tetherin is an exosomal tether.eLife · 2016Article
- GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Vibrio vulnificus VvhA induces autophagy-related cell death through the lipid raft-dependent c-Src/NOX signaling pathway.Scientific reports · 2016Article
- Red Blood Cell Susceptibility to Pneumolysin: CORRELATION WITH MEMBRANE BIOCHEMICAL AND PHYSICAL PROPERTIES.The Journal of biological chemistry · 2016Article
- Recent progress on lipid lateral heterogeneity in plasma membranes: From rafts to submicrometric domains.Progress in lipid research · 2016Review
- Cholesterol segregates into submicrometric domains at the living erythrocyte membrane: evidence and regulation.Cellular and molecular life sciences : CMLS · 2015Article
- Association between Obesity and Cervical Microflora Dominated by Lactobacillus iners in Korean Women.Journal of clinical microbiology · 2015Article
- More Than a Pore: The Interplay of Pore-Forming Proteins and Lipid Membranes.The Journal of membrane biology · 2015Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is increasing evidence that sphingolipid- and cholesterol-rich microdomains (rafts) exist in the plasma membrane. Specific proteins assemble in these membrane domains and play a role in signal transduction and many other cellular events. Cholesterol depletion causes disassembly of the raft-associated proteins, suggesting an essential role of cholesterol in the structural maintenance and function of rafts. However, no tool has been available for the detection and monitoring of raft cholesterol in living cells. Here we show that a protease-nicked and biotinylated derivative (BCtheta) of perfringolysin O (theta-toxin) binds selectively to cholesterol-rich microdomains of intact cells, the domains that fulfill the criteria of rafts. We fractionated the homogenates of nontreated and Triton X-100-treated platelets after incubation with BCtheta on a sucrose gradient. BCtheta was predominantly localized in the floating low-density fractions (FLDF) where cholesterol, sphingomyelin, and Src family kinases are enriched. Immunoelectron microscopy demonstrated that BCtheta binds to a subpopulation of vesicles in FLDF. Depletion of 35% cholesterol from platelets with cyclodextrin, which accompanied 76% reduction in cholesterol from FLDF, almost completely abolished BCtheta binding to FLDF. The staining patterns of BCtheta and filipin in human epidermoid carcinoma A431 cells with and without cholesterol depletion suggest that BCtheta binds to specific membrane domains on the cell surface, whereas filipin binding is indiscriminate to cell cholesterol. Furthermore, BCtheta binding does not cause any damage to cell membranes, indicating that BCtheta is a useful probe for the detection of membrane rafts in living cells.
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