ArticleTransgenic research2001
Sorting of glycoprotein B from human cytomegalovirus to protein storage vesicles in seeds of transgenic tobacco.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Glyco-Engineering of Plant-Based Expression Systems.Advances in biochemical engineering/biotechnology · 2021Article
- Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO andInternational journal of molecular sciences · 2019Article
- Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco.BMC biotechnology · 2011Article
- Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis.Plant physiology · 2011Article
- Expression and subcellular targeting of human insulin-like growth factor binding protein-3 in transgenic tobacco plants.Transgenic research · 2009Article
- A green fluorescent protein fused to rice prolamin forms protein body-like structures in transgenic rice.Journal of experimental botany · 2009Article
- Sustained expression of human cytomegalovirus glycoprotein B (UL55) in the seeds of homozygous rice plants.Molecular biotechnology · 2008Article
- Article
- The Quest to Understand the Basis and Mechanisms that Control Expression of Introduced Transgenes in Crop Plants.Plant signaling & behavior · 2006Article
- The intracellular fate of a recombinant protein is tissue dependent.Plant physiology · 2006Article
- Human CD14 expressed in seeds of transgenic tobacco displays similar proteolytic resistance and bioactivity with its mammalian-produced counterpart.Transgenic research · 2006Article
- Plant cell factories and mucosal vaccines.Current opinion in biotechnology · 2003Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As part of ongoing studies into the use of plant expression systems for making human therapeutic proteins, we have successfully expressed the major glycoprotein, gB, of human cytomegalovirus (HCMV) in transgenic tobacco plants. Viral glycoprotein was detectable in the protein extracts of mature tobacco seeds using neutralizing and non-neutralizing monoclonal antibodies specific for gB. Although several mammalian proteins have been expressed in tobacco, localization of these proteins in transgenic tobacco tissue has not been extensively examined. The objective of this study was to identify the site(s) of recombinant gB deposition in mature tobacco seeds. Using immunogold labelling and electron microscopy, we found specific labelling for gB in the endosperm of transgenic seeds, with gB localized almost exclusively in protein storage vesicles (PSV). This occurred in seeds that were freshly harvested and in seeds that had been stored for several months. These data indicate that gB behaves like a plant storage protein when expressed in tobacco seeds, and provide further support for the suitability of plants for producing recombinant proteins of potential clinical relevance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.