ArticleThe Korean journal of parasitology2001
Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis.
Article in The Korean journal of parasitology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- One-pot nanoflower-based sensitive colorimetric biosensor for multihost detection of zoonotic clonorchiasis.PLoS neglected tropical diseases · 2026Article
- A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay.PLoS neglected tropical diseases · 2024Article
- Evaluation of Crude and Recombinant Antigens ofDiagnostics (Basel, Switzerland) · 2023Article
- Combination of Biodata Mining and Computational Modelling in Identification and Characterization of ORF1ab Polyprotein of SARS-CoV-2 Isolated from Oronasopharynx of an Iranian Patient.Biological procedures online · 2020Article
- Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide.PLoS neglected tropical diseases · 2018Article
- Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target.International journal of molecular sciences · 2017Article
- Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production.Parasites & vectors · 2017Article
- Development of "-omics" research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis.Frontiers in microbiology · 2014Review
- Human antibody response to thioredoxin peroxidase-1 and tandem repeat proteins as immunodiagnostic antigen candidates for Schistosoma japonicum infection.The American journal of tropical medicine and hygiene · 2011Article
- Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis.Parasitology research · 2011Article
- Functional genes and proteins of Clonorchis sinensis.The Korean journal of parasitology · 2009Review
- Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1alpha.Parasitology research · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.
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