ArticlePlant physiology2001
Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host Nicotiana attenuata. IV. Insect-Induced ethylene reduces jasmonate-induced nicotine accumulation by regulating putrescine N-methyltransferase transcripts.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 231 citations in OpenAlex.
- Ethylene represses jasmonate signaling to attenuate nicotine biosynthesis inFrontiers in plant science · 2026Article
- A New Model of the Mechanisms behind Glucose Oxidase Action in Plant Insect Interactions.Journal of chemical ecology · 2025Review
- Ethylene signaling is essential for mycorrhiza-induced resistance against chewing herbivores in tomato.Journal of experimental botany · 2025Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- High-quality chromosome-level scaffolds of the plant bug Pachypeltis micranthus provide insights into the availability of Mikania micrantha control.BMC genomics · 2023Article
- Transcriptomic analysis provides insights into the AUXIN RESPONSE FACTOR 6-mediated repression of nicotine biosynthesis in tobacco (Nicotiana tabacum L.).Plant molecular biology · 2021Article
- Ethylene response factors 15 and 16 trigger jasmonate biosynthesis in tomato during herbivore resistance.Plant physiology · 2021Article
- Information theory tests critical predictions of plant defense theory for specialized metabolism.Science advances · 2020Article
- Oral cues are not enough: induction of defensive proteins in Nicotiana tabacum upon feeding by caterpillars.Planta · 2020Article
- Mutually Regulated AP2/ERF Gene Clusters Modulate Biosynthesis of Specialized Metabolites in Plants.Plant physiology · 2020Article
- Plant Defense Responses Induced by Two Herbivores and Consequences for WhiteflyFrontiers in physiology · 2019Article
- Fully Bayesian analysis of RNA-seq counts for the detection of gene expression heterosis.Journal of the American Statistical Association · 2019Article
- A mechanism for sequence specificity in plant-mediated interactions between herbivores.The New phytologist · 2017Article
- Stability of Single-Parent Gene Expression Complementation in Maize Hybrids upon Water Deficit Stress.Plant physiology · 2017Article
- Tropane and Granatane Alkaloid Biosynthesis: A Systematic Analysis.Molecules (Basel, Switzerland) · 2016Review
- Alternative splicing of basic chitinase gene PR3b in the low-nicotine mutants of Nicotiana tabacum L. cv. Burley 21.Journal of experimental botany · 2016Article
- Auxin Is Rapidly Induced by Herbivore Attack and Regulates a Subset of Systemic, Jasmonate-Dependent Defenses.Plant physiology · 2016Article
- How plants handle multiple stresses: hormonal interactions underlying responses to abiotic stress and insect herbivory.Plant molecular biology · 2016Review
- Transgenic analysis reveals LeACS-1 as a positive regulator of ethylene-induced shikonin biosynthesis in Lithospermum erythrorhizon hairy roots.Plant molecular biology · 2016Article
- Identification of Topping Responsive Proteins in Tobacco Roots.Frontiers in plant science · 2016Article
16 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
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Abstract
Attack by the specialist herbivore, Manduca sexta, on its native host Nicotiana attenuata Torr. ex Wats. produces a dramatic ethylene release, a jasmonate burst, and a suppression of the nicotine accumulation that results from careful simulations of the herbivore's damage. Methyl-jasmonate (MeJA) treatment induces nicotine biosynthesis. However, this induction can be suppressed by ethylene as pretreatment of plants with 1-methylcyclopropene (1-MCP), a competitive inhibitor of ethylene receptors, restores the full MeJA-induced nicotine response in herbivore attacked plants (J. Kahl, D.H. Siemens, R.J. Aerts, R. Gäbler, F. Kühnemann, C.A. Preston, I.T. Baldwin [2000] Planta 210: 336-342). To understand whether this herbivore-induced signal cross-talk occurs at the level of transcript accumulation, we cloned the putrescine methyltransferase genes (NaPMT1 and NaPMT2) of N. attenuata, which are thought to represent the rate limiting step in nicotine biosynthesis, and measured transcript accumulations by northern analysis after various jasmonate, 1-MCP, ethephon, and herbivory treatments. Transcripts of both root putrescine N-methyltransferase (PMT) genes and nicotine accumulation increased dramatically within 10 h of shoot MeJA treatment and immediately after root treatments. Root ethephon treatments suppressed this response, which could be reversed by 1-MCP pretreatment. Moreover, 1-MCP pretreatment dramatically amplified the transcript accumulation resulting from both wounding and M. sexta herbivory. We conclude that attack from this nicotine-tolerant specialist insect causes N. attenuata to produce ethylene, which directly suppresses the nitrogen-intensive biosynthesis of nicotine.
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