ArticlePlant physiology2001
Aspartate kinase 2. A candidate gene of a quantitative trait locus influencing free amino acid content in maize endosperm.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 51 citations in OpenAlex.
- Unveiling the Link Between Expression of Aspartate Kinase2 (ask2) Gene and Accumulation of Methionine in Developing Maize Kernels.Biochemical genetics · 2026Article
- Breeding for colored quality protein popcorn with improved amino acid composition.Frontiers in plant science · 2026Article
- Opaque-2 induced zein reduction and lysine increase suggest a route to quality protein sweet corn.Frontiers in plant science · 2026Article
- Editing the 19 kDa alpha-zein gene family generates non-opaque2-based quality protein maize.Plant biotechnology journal · 2024Article
- Assessing sequence variation, haplotype analysis and molecular characterisation of aspartate kinase2 (ask2) gene regulating methionine biosynthesis in diverse maize inbreds.Molecular genetics and genomics : MGG · 2024Article
- Genetic enhancement of essential amino acids for nutritional enrichment of maize protein quality through marker assisted selection.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- The genetic architecture of amino acids dissection by association and linkage analysis in maize.Plant biotechnology journal · 2017Article
- Identification of the quantitative trait loci (QTL) underlying water soluble protein content in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis.Planta · 2011Article
- Compositional and transcriptional analyses of reduced zein kernels derived from the opaque2 mutation and RNAi suppression.Plant molecular biology · 2010Article
- Threonine-insensitive homoserine dehydrogenase from soybean: genomic organization, kinetic mechanism, and in vivo activity.The Journal of biological chemistry · 2010Article
- Seed-specific expression of truncated OsGAD2 produces GABA-enriched rice grains that influence a decrease in blood pressure in spontaneously hypertensive rats.Transgenic research · 2009Article
- Quantitative trait loci controlling sulfur containing amino acids, methionine and cysteine, in soybean seeds.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- An improved genetic model generates high-resolution mapping of QTL for protein quality in maize endosperm.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Quantitative trait locus mapping of loci influencing elongation factor 1alpha content in maize endosperm.Plant physiology · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maize (Zea mays) Oh545o2 inbred accumulates an exceptionally high level of free amino acids, especially lysine (Lys), threonine (Thr), methionine, and iso-leucine. In a cross between Oh545o2 and Oh51Ao2, we identified several quantitative trait loci linked with this phenotype. One of these is on the long arm of chromosome 2 and is linked with loci encoding aspartate (Asp) kinase 2 and Asp kinase (AK)-homoserine dehydrogenase (HSDH) 2. To investigate whether these enzymes can contribute to the high levels of Asp family amino acids, we measured their specific activity and feedback inhibition properties, as well as activities of several other key enzymes involved in Lys metabolism. We did not find a significant difference in total activity of dihydrodipicolinate synthase, HSDH, and Lys ketoglutarate reductase between these inbreds, and the feedback inhibition properties of HSDH and dihyrodipicolinate synthase by Lys and/or Thr were similar. The most significant difference we found between Oh545o2 and Oh51Ao2 is feedback inhibition of AK by Lys but not Thr. AK activity in Oh545o2 is less sensitive to Lys inhibition than that in Oh51Ao2, with a Lys I50 twice that of Oh51Ao2. AK activity in Oh545o2 endosperm is also higher than in Oh51Ao2 at 15 d after pollination, but not 20 d after pollination. The results indicate that the Lys-sensitive Asp kinase 2, rather than the Thr-sensitive AK-HSDH2, is the best candidate gene for the quantitative trait locus affecting free amino acid content in Oh545o2.
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