ArticlePlant physiology2001
Genetic analysis of amino acid accumulation in opaque-2 maize endosperm.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 119 citations in OpenAlex.
- Unveiling the Link Between Expression of Aspartate Kinase2 (ask2) Gene and Accumulation of Methionine in Developing Maize Kernels.Biochemical genetics · 2026Article
- Meta-QTLs and candidate genes for kernel protein content in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- Genetic analysis of QTLs for lysine content in four maize DH populations.BMC genomics · 2024Article
- Genetic and molecular understanding for the development of methionine-rich maize: a holistic approach.Frontiers in plant science · 2023Review
- Acrylamide in Corn-Based Thermally Processed Foods: A Review.Journal of agricultural and food chemistry · 2022Review
- Multiomics approach reveals a role of translational machinery in shaping maize kernel amino acid composition.Plant physiology · 2022Article
- Expression analysis of3 Biotech · 2021Article
- The Arbuscular Mycorrhizal FungusFrontiers in plant science · 2021Article
- Genomic Prediction Informed by Biological Processes Expands Our Understanding of the Genetic Architecture Underlying Free Amino Acid Traits in DryG3 (Bethesda, Md.) · 2020Article
- Temporal profiling of essential amino acids in developing maize kernel of normal,Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Nitrogen Supply Drives Senescence-Related Seed Storage Protein Expression in Rapeseed Leaves.Genes · 2019Article
- Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.The Plant cell · 2015Article
- Mapping QTLs for opaque2 modifiers influencing the tryptophan content in quality protein maize using genomic and candidate gene-based SSRs of lysine and tryptophan metabolic pathway.Plant cell reports · 2015Article
- Transcriptional network analysis of the tryptophan-accumulating rice mutant during grain filling.Molecular genetics and genomics : MGG · 2012Article
- The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses.BMC genomics · 2011Article
- Compositional and transcriptional analyses of reduced zein kernels derived from the opaque2 mutation and RNAi suppression.Plant molecular biology · 2010Article
- Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency.Plant cell reports · 2010Article
- Developmental analysis of maize endosperm proteome suggests a pivotal role for pyruvate orthophosphate dikinase.Plant physiology · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545o2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545o2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51Ao2 and W64Ao2, respectively. We crossed Oh545o2 to Oh51Ao2 and analyzed the F(2:3) progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 is coincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA in opaque-2 mutants.
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