ArticleProceedings of the National Academy of Sciences of the United States of America2001
Direct transformation of rodent fibroblasts by jaagsiekte sheep retrovirus DNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 150 citations in OpenAlex.
- Milk Transmission of Mammalian Retroviruses.Microorganisms · 2023Review
- Differences in CpG island distribution between exogenous and endogenous jaagsiekte sheep retrovirus strains.Veterinary research forum : an international quarterly journal · 2023Article
- Neoplasia-Associated Wasting Diseases with Economic Relevance in the Sheep Industry.Animals : an open access journal from MDPI · 2021Review
- Enzootic nasal tumor virus type 2 envelope of goats acts as a retroviral oncogene in cell transformation.Virus genes · 2021Article
- Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.Journal of virology · 2019Article
- Ovine Pulmonary Adenocarcinoma: A Unique Model to Improve Lung Cancer Research.Frontiers in oncology · 2019Review
- SAMHD1 inhibits epithelial cell transformation in vitro and affects leukemia development in xenograft mice.Cell cycle (Georgetown, Tex.) · 2018Article
- A human endogenous retrovirus-derived gene that can contribute to oncogenesis by activating the ERK pathway and inducing migration and invasion.PLoS pathogens · 2017Article
- Evidence against a role for jaagsiekte sheep retrovirus in human lung cancer.Retrovirology · 2017Article
- Large area magnetic micropallet arrays for cell colony sorting.Lab on a chip · 2016Article
- Role for a Zinc Finger Protein (Zfp111) in Transformation of 208F Rat Fibroblasts by Jaagsiekte Sheep Retrovirus Envelope Protein.Journal of virology · 2015Article
- Recent advances in the study of active endogenous retrovirus envelope glycoproteins in the mammalian placenta.Virologica Sinica · 2015Review
- Jaagsiekte sheep retrovirus infection of lung slice cultures.Retrovirology · 2015Article
- The sheep tetherin paralog oBST2B blocks envelope glycoprotein incorporation into nascent retroviral virions.Journal of virology · 2015Article
- The cytoplasmic tail of retroviral envelope glycoproteins.Progress in molecular biology and translational science · 2015Review
- "Ménage à Trois": the evolutionary interplay between JSRV, enJSRVs and domestic sheep.Viruses · 2014Review
- Jaagsiekte sheep retrovirus detected in human lung cancer tissue arrays.BMC research notes · 2014Article
- Human endogenous retrovirus-K(II) envelope induction protects neurons during HIV/AIDS.PloS one · 2014Article
- Host species barriers to Jaagsiekte sheep retrovirus replication and carcinogenesis.Journal of virology · 2013Article
- Human T lymphotropic virus type 1 SU residue 195 plays a role in determining the preferential CD4+ T cell immortalization/transformation tropism.Journal of virology · 2013Article
18 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
Abstract
Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary carcinoma, a unique animal model for human bronchioalveolar carcinoma. We previously isolated a JSRV proviral clone and showed that it was both infectious and oncogenic. Thus JSRV is necessary and sufficient for the development of ovine pulmonary carcinoma, but no data are available on the mechanisms of transformation. Inspection of the JSRV genome reveals standard retroviral genes, but no evidence for a viral oncogene. However, an alternate ORF in pol (orf-x) might be a candidate for a transforming gene. We tested whether the JSRV genome might encode a transforming gene by transfecting an expression plasmid for JSRV [pCMVJS21, driven by the cytomegalovirus (CMV) immediate early promoter] into mouse NIH 3T3 cells. Foci of transformed cells appeared in the transfected cultures 2-3 weeks posttransfection; cloned transformants showed anchorage independence for growth, and they expressed JSRV RNA. These results indicate that the JRSV genome contains information with direct transforming potential for NIH 3T3 cells. Transfection of a mutated version of pCMVJS21 in which the orf-x protein was terminated by two stop codons also gave transformed foci. Thus, orf-x was eliminated as the candidate transforming gene. In addition, another derivative of pCMVJS21 (pCMVJS21DeltaGP) in which the gag, pol (and orf-x) coding sequences were deleted also gave transformed foci. These results indicate that the envelope gene carries the transforming potential. This is an unusual example of a native retroviral structural protein with transformation potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.