ArticleThe EMBO journal2001
A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed.
- Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naïve chromosomal locus.Nucleus (Austin, Tex.) · 2025Article
- Genes and genetics belong to maize haploid induction.Frontiers in plant science · 2025Review
- Synthetic maize centromeres transmit chromosomes across generations.Nature plants · 2023Article
- Epigenetic control of centromere: what can we learn from neocentromere?Genes & genomics · 2022Review
- Case Report: Prenatal Identification of aFrontiers in genetics · 2022Article
- Formation of artificial chromosomes in Caenorhabditis elegans and analyses of their segregation in mitosis, DNA sequence composition and holocentromere organization.Nucleic acids research · 2021Article
- Chromosomal variations of Lycoris species revealed by FISH with rDNAs and centromeric histone H3 variant associated DNAs.PloS one · 2021Article
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- Article
- Profiling of LINE-1-Related Genes in Hepatocellular Carcinoma.International journal of molecular sciences · 2019Review
- The dark side of centromeres: types, causes and consequences of structural abnormalities implicating centromeric DNA.Nature communications · 2018Review
- Posttranslational modifications of CENP-A: marks of distinction.Chromosoma · 2018Review
- Review
- Structure and Stability of Telocentric Chromosomes in Wheat.PloS one · 2015Article
- The centromere: epigenetic control of chromosome segregation during mitosis.Cold Spring Harbor perspectives in biology · 2014Review
- Neocentromeres: a place for everything and everything in its place.Trends in genetics : TIG · 2014Review
- Review
- Kinetochores coordinate pericentromeric cohesion and early DNA replication by Cdc7-Dbf4 kinase recruitment.Molecular cell · 2013Article
- Chromosome engineering allows the efficient isolation of vertebrate neocentromeres.Developmental cell · 2013Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromere protein A (CENP-A) is an essential centromere-specific histone H3 homologue. Using combined chromatin immunoprecipitation and DNA array analysis, we have defined a 330 kb CENP-A binding domain of a 10q25.3 neocentromere found on the human marker chromosome mardel(10). This domain is situated adjacent to the 80 kb region identified previously as the neocentromere site through lower-resolution immunofluorescence/FISH analysis of metaphase chromosomes. The 330 kb CENP-A binding domain shows a depletion of histone H3, providing evidence for the replacement of histone H3 by CENP-A within centromere-specific nucleosomes. The DNA within this domain has a high AT-content comparable to that of alpha-satellite, a high prevalence of LINEs and tandem repeats, and fewer SINEs and potential genes than the surrounding region. FISH analysis indicates that the normal 10q25.3 genomic region replicates around mid-S phase. Neocentromere formation is accompanied by a replication time lag around but not within the CENP-A binding region, with this lag being significantly more prominent to one side. The availability of fully sequenced genomic markers makes human neocentromeres a powerful model for dissecting the functional domains of complex higher eukaryotic centromeres.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.