ArticleThe EMBO journal2001
MOT1-catalyzed TBP-DNA disruption: uncoupling DNA conformational change and role of upstream DNA.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Structural basis for TBP displacement from TATA box DNA by the Swi2/Snf2 ATPase Mot1.Nature structural & molecular biology · 2023Article
- Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.Nucleic acids research · 2019Article
- Article
- Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.The Journal of biological chemistry · 2016Article
- Article
- Mot1 redistributes TBP from TATA-containing to TATA-less promoters.Molecular and cellular biology · 2013Article
- Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.The Journal of biological chemistry · 2012Article
- One small step for Mot1; one giant leap for other Swi2/Snf2 enzymes?Biochimica et biophysica acta · 2011Review
- Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP.Nature · 2011Article
- Elucidating the mechanism of DNA-dependent ATP hydrolysis mediated by DNA-dependent ATPase A, a member of the SWI2/SNF2 protein family.Nucleic acids research · 2009Article
- TATA-binding protein variants that bypass the requirement for Mot1 in vivo.The Journal of biological chemistry · 2009Article
- Function and structural organization of Mot1 bound to a natural target promoter.The Journal of biological chemistry · 2008Article
- Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA.The EMBO journal · 2006Article
- Snf1-dependent and Snf1-independent pathways of constitutive ADH2 expression in Saccharomyces cerevisiae.Genetics · 2006Article
- Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants.Nucleic acids research · 2005Article
- High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription.The EMBO journal · 2003Article
- Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme.Molecular and cellular biology · 2003Article
- A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.Molecular and cellular biology · 2002Article
- Mot1 associates with transcriptionally active promoters and inhibits association of NC2 in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
SNF2/SWI2-related ATPases employ ATP hydrolysis to disrupt protein-DNA interactions, but how ATP hydrolysis is coupled to disruption is not understood. Here we examine the mechanism of action of MOT1, a yeast SNF2/SWI2-related ATPase that uses ATP hydrolysis to remove TATA binding protein (TBP) from DNA. MOT1 function requires a 17 bp DNA 'handle' upstream of the TATA box, which must be double stranded. Remarkably, MOT1-catalyzed disruption of TBP-DNA does not appear to require DNA strand separation, DNA bending or twisting of the DNA helix. Thus, TBP-DNA disruption is accomplished in a reaction apparently not driven by a change in DNA structure. MOT1 action is supported by DNA templates in which the handle is connected to the TATA box via single-stranded DNA, indicating that the upstream duplex DNA can be conformationally uncoupled from the TATA box. Combining these results with proposed similarities between SNF2/SWI2 ATPases and helicases, we suggest that MOT1 uses ATP hydrolysis to translocate along the handle and thereby disrupt interactions between TBP and DNA.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.