ArticleMolecular biology of the cell2001
Profilin binding to poly-L-proline and actin monomers along with ability to catalyze actin nucleotide exchange is required for viability of fission yeast.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 151 citations in OpenAlex.
- Localization and functional exploration of leiomodin-2's C-terminal binding sites.Biochimica et biophysica acta. Proteins and proteomics · 2026Article
- Generation of a functional fluorescently-tagged PFN-3 profilin transgene inmicroPublication biology · 2026Article
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- A revised model of nuclear actin import: Importin 9 competes with cofilin, profilin, and RanGTP for actin binding.bioRxiv : the preprint server for biology · 2025Article
- Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes.eLife · 2025Article
- Membrane-associated polymerases deliver most of the actin subunits to a lamellipodial network.bioRxiv : the preprint server for biology · 2025Article
- Interaction of cardiac leiomodin with the native cardiac thin filament.PLoS biology · 2025Article
- Article
- Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins.European journal of cell biology · 2023Article
- Pointed-end processive elongation of actin filaments byScience advances · 2022Article
- A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity.Communications biology · 2022Article
- Actin assembly requirements of the formin Fus1 to build the fusion focus.Journal of cell science · 2022Article
- Allosteric regulation controls actin-bundling properties of human plastins.Nature structural & molecular biology · 2022Article
- Biochemical characterization of actin assembly mechanisms with ALS-associated profilin variants.European journal of cell biology · 2022Article
- Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.Cells · 2021Article
- Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.Biophysical journal · 2021Article
- Variable Autoinhibition among Deafness-Associated Variants of Diaphanous 1 (DIAPH1).Biochemistry · 2021Article
- Article
- Competition for delivery of profilin-actin to barbed ends limits the rate of formin-mediated actin filament elongation.The Journal of biological chemistry · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
We tested the ability of 87 profilin point mutations to complement temperature-sensitive and null mutations of the single profilin gene of the fission yeast Schizosaccharomyces pombe. We compared the biochemical properties of 13 stable noncomplementing profilins with an equal number of complementing profilin mutants. A large quantitative database revealed the following: 1) in a profilin null background fission yeast grow normally with profilin mutations having >10% of wild-type affinity for actin or poly-L-proline, but lower affinity for either ligand is incompatible with life; 2) in the cdc3-124 profilin ts background, fission yeast function with profilin having only 2-5% wild-type affinity for actin or poly-L-proline; and 3) special mutations show that the ability of profilin to catalyze nucleotide exchange by actin is an essential function. Thus, poly-L-proline binding, actin binding, and actin nucleotide exchange are each independent requirements for profilin function in fission yeast.
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