ArticleMolecular biology of the cell2001
Depletion of acyl-coenzyme A-binding protein affects sphingolipid synthesis and causes vesicle accumulation and membrane defects in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 135 citations in OpenAlex.
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Structure of the yeast ceramide synthase.Nature structural & molecular biology · 2025Article
- Membrane fluidity control by the Magnaporthe oryzae acyl-CoA binding protein sets the thermal range for host rice cell colonization.PLoS pathogens · 2024Article
- Acyl-coenzyme A binding protein MoAcb1 regulates conidiation and pathogenicity inFrontiers in microbiology · 2023Article
- From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI.Trends in endocrinology and metabolism: TEM · 2021Review
- The diversity of ACBD proteins - From lipid binding to protein modulators and organelle tethers.Biochimica et biophysica acta. Molecular cell research · 2020Review
- Advances in Understanding the Acyl-CoA-Binding Protein in Plants, Mammals, Yeast, and Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2020Review
- Functional characterization of acyl-CoA binding protein in Neospora caninum.Parasites & vectors · 2020Article
- Expression of acyl-CoA-binding protein 5 from Rhodnius prolixus and its inhibition by RNA interference.PloS one · 2020Article
- HIF-1-dependent regulation of lifespan inAging · 2017Article
- Regulation of very-long acyl chain ceramide synthesis by acyl-CoA-binding protein.The Journal of biological chemistry · 2017Article
- Deficiency of a Retinal Dystrophy Protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), Impairs Peroxisomal β-Oxidation of Very-long-chain Fatty Acids.The Journal of biological chemistry · 2017Article
- Deciphering the roles of acyl-CoA-binding proteins in plant cells.Protoplasma · 2016Review
- Secreted Acb1 Contributes to the Yeast-to-Hypha Transition in Cryptococcus neoformans.Applied and environmental microbiology · 2016Article
- Compromised epidermal barrier stimulates Harderian gland activity and hypertrophy in ACBP-/- mice.Journal of lipid research · 2015Article
- Minireview: translocator protein (TSPO) and steroidogenesis: a reappraisal.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.The Plant journal : for cell and molecular biology · 2015Article
- Arabidopsis cytosolic acyl-CoA-binding proteins ACBP4, ACBP5 and ACBP6 have overlapping but distinct roles in seed development.Bioscience reports · 2014Article
- Production of a Brassica napus Low-Molecular Mass Acyl-Coenzyme A-Binding Protein in Arabidopsis Alters the Acyl-Coenzyme A Pool and Acyl Composition of Oil in Seeds.Plant physiology · 2014Article
- Acyl-CoA metabolism and partitioning.Annual review of nutrition · 2014Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deletion of the yeast gene ACB1 encoding Acb1p, the yeast homologue of the acyl-CoA-binding protein (ACBP), resulted in a slower growing phenotype that adapted into a faster growing phenotype with a frequency >1:10(5). A conditional knockout strain (Y700pGAL1-ACB1) with the ACB1 gene under control of the GAL1 promoter exhibited an altered acyl-CoA profile with a threefold increase in the relative content of C18:0-CoA, without affecting total acyl-CoA level as previously reported for an adapted acb1Delta strain. Depletion of Acb1p did not affect the general phospholipid pattern, the rate of phospholipid synthesis, or the turnover of individual phospholipid classes, indicating that Acb1p is not required for general glycerolipid synthesis. In contrast, cells depleted for Acb1p showed a dramatically reduced content of C26:0 in total fatty acids and the sphingolipid synthesis was reduced by 50-70%. The reduced incorporation of [(3)H]myo-inositol into sphingolipids was due to a reduced incorporation into inositol-phosphoceramide and mannose-inositol-phosphoceramide only, a pattern that is characteristic for cells with aberrant endoplasmic reticulum to Golgi transport. The plasma membrane of the Acb1p-depleted strain contained increased levels of inositol-phosphoceramide and mannose-inositol-phosphoceramide and lysophospholipids. Acb1p-depleted cells accumulated 50- to 60-nm vesicles and autophagocytotic like bodies and showed strongly perturbed plasma membrane structures. The present results strongly suggest that Acb1p plays an important role in fatty acid elongation and membrane assembly and organization.
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